The Resistant Reaction Of Kidney Bean And Tomato Induced By Jasmonic Acid To Liriomyza Sativae Blanchard

Liriomyza sativae Blanchard is an important pest of vegetables. Jasmonic acid(JA) is aplant hormone that plays an important role in the induction of plant resistance to insects. Toclarify inducement of exogenous JA on resistance of kidney bean to L. sativae, kidney beanand tomato were taken as material and biometrics and defense enzyme activities (POD, PPOand LOX) were measured. The main results are as follows:1. The feeding and oviposition selectivity, developmental duration of eggs and larva,feeding area and pupa weight were significantly influenced on JA-induced kidney beanseedlings and the influence was related to the JA concentration. The fecundity was19nymphless than that of control at the concentration of0.1mmol/L of jasmonic acid. The resistance ofkidney bean against L. sativae was significantly enhanced at the concentration of0.1and1mmol/L of a jasmonic solution.0.001and0.01mmol/L JA only have a significant effect forthe duration of larval development.2. Applying exogenous JA on kidney bean leaves reduced feeding selectivity of adultdevelopment of eggs and larvae. This effect was significantly related to time span of JAtreatment.0.1mmol/L JA treatment within24h had no effect on adult feeding, but theinduction of resistance was enhanced after72and120h. It was different to the adult, theeffect of JA to the development of larva enhanced significantly in6to24h and is not obviousafter72and120h.3. Exogenous JA treatment resulted in an increase of PPO, POD and LOX activities, butthe effect was significantly related to JA concentration and time span of JA treatment. Theinductive effect of three defensive enzymes in leaves of kidney bean was not obvious at0.001mmol/L JA, but significant at0.1mmol/L JA. For activities of PPO and POD in tomato leaves,the inductive effect of four JA concentrations were all significant. However, effect was notsignificant for LOX at0.001and1mmol/L JA. In addition, the strongest inductive effect wasdetected after72h of0.1mmol/L JA treatment for LOX in tomato leaves, but after24h forPOD and PPO in tomato leaves as well as POD, PPO and LOX in kidney bean leaves. 4. The activities of PPO and POD in kidney bean leaves was negative correlation tofeeding and oviposition selectivity of adult, pupal weight and feeding area per larva, andpositive correlation to developmental duration of eggs and larvae. PPO activity was mostcorrelated with the resistance increase, followed by POD, but the relationship between LOXand resistance was not significant.