Drought Resistance Of CBF1and KatG Transgenic Cotton

Objective: Xinjiang is China’s most suitable for the cultivation of cotton regions.But Xinjiang in the Asian continent, due to the seasonal drought, or is the uncertaintyof the effects of drought, drought worsening, affecting the cotton yield in Xinjiang. Sothe development of drought-resistant GM cotton is essential.Before the study, already get CBF1and KatG gene and high cotton. CBF1genecomes from Glenn food. It is the ability of a transcriptional Activator gene, the mainfunction is to control the gene expression of a series of drought resistance, coldresistance, thereby increasing the capacity of plant stress resistance. KatG gene fromEscherichia coli. It can produce catalase in vivo in animals and plants a CAT one ofthe members of the gene family, main function of catalase is encoded, on droughtresistance of plant defense, has a very big role. This study verified by transgenicmeans improved cotton varieties to improve the ability of cotton resistance to stressand ultimately increase cotton production.Methods: CBF1and KatG gene of high generation cotton material. Grown in thefield, after kanamycin and screening PCR-positive plant, through germination rate andchlorophyll content in seedling stage and the seedling, bud and flower bud stage andduring the flowering period of drought treatment related to photosynthesis,chlorophyll fluorescence parameters and drought-resistance physiological andbiochemical indexes and agronomic studies.Results：(1) Germination rates in both transgenic cotton78%, and chlorophyll content inseedling increased significantly. Description: a genetically modified cotton producemore organic matter, to increase cotton production.(2) Stress on photosynthesis and chlorophyll fluorescence parameters of valueafter comparative analysis: Under different drought treatments, CBF1and florescenceof KatG gene cotton bud stage and net photosynthetic rate (Pn), stomatal conductance(Gs), intercellular CO2concentration (Ci) and the maximum photochemical efficiencyof photosystem Ⅱ (Fv/Fm) values higher than those of control. Description oftransgenic cotton eased the drought stress on cotton photosynthesis inhibition,suggested that transgenic cotton could increase drought resistance and yield.(3) Comparison and analysis of physiological indicators of stress in transgeniccotton: Under different drought stress, electricalconductivity, malonaldehyde, proline and soluble sugar content higher. To KatG gene cotton of catalase, peroxidase andsuperoxide dismutase activity analysis, under drought stress, transgenic cotton withKatG gene on the three enzyme activity was significantly higher. Descriptiontransgenic cotton to overcome negative factors, improve tolerance.(4) Stress for plant height, diameter, number of leaves, relative water content,biomass, number of fruit branches, and yield boll comparative analysis of agronomictraits: Under drought stress, in addition to outside the cotton boll comparison, twotransgenic cotton agronomic quantitative indicators were higher than controls. Twotransgenic cotton in drought tolerance capacity after drought treatment significantlyincreased cotton production increased significantly.Conclusion: Under drought stress, KatG gene and CBF1in photosynthesis,chlorophyll fluorescence parameters of cotton, physiological and agronomic aspectshave shown excellent growth and physiological advantages. Description capacity canincrease stress resistance of transgenic cotton, and increases the yield of transgeniccotton.