Genetic Differentiation Of Geographical Distribution Wild Soybean In Shanxi Province

Subgenus Glycine and Subgenus Soja were included in Glycine.Subgenus soja contains cultivated soybean (Glycine max (L.) Merr.) and annual wild soybean (Glycine soja Sieb.&Zucc.). There is an easier hybridization between them and a good fecundity in their filial generations. In light of this, wild soybean with a high genetic diversity can be used to broaden the genetic base of cultivated soybean.Located in the west of Taihang mountains in the east of the Yellow River, Shanxi has a unique environment for the growth of wild soybean.According to Vavilov’s theory of eight crop origin centers, soybean originates from mountainous regions and the adjacent areas in the central and western part of China.Shanxi is in the area.To date, few reports have been made upon the researches of wild soybean in Shanxi. A comprehensive analysis of wild soybean’s genetic diversity in the area can provide some basic information for the germplasm conservation and molecular breeding in the future.This study choosed36primers to evaluate genetic diversity of319wild soybeans and landraces. Several conclusions would be drawn from this research as follows:1.389alleles were identified.In wild soybeans,352alleles were identified,with5-16allelesper primer and averaged9.778. In landraces,311alleles were identified,with1-14alleles per primer and averaged8.639.The average alleles, the average Shannon-Weaver indices and the average polymorphic information content (PIC) in wild soybeans were higher than landraces.Wild soybeans and landraces were formed into two separate genetic groups, by STRUCTURE analysis.2.229wild soybeans were divided into different groups according geographical orgin.In view of the average Shannon-Weaver indices of wild soybeans, Taiyuan owned mostabundant(1.553), the lowest regions were Shuozhou(0.9023). The genetic distance, between Jingzhong and Changzhi,is shortest.Between Linfen and Shuozhou,the genetic distance is longest.The results,from clustering analysis between groups and individual principal component analysis,are basically the same. The research also found that the genetic background and population structure of wild soybean were strongly affected by geographical orgin.3. As to90landraces, Xinzhou showed the highest level of genetic diversity, and Shuozhou presented the lowest genetic diversity. The genetic distance, between Jingzhong and Lvliang,is shortest. The results,from clustering analysis between groups and individual principal component analysis,are basically the same.