Identification Of Molecular Marker In Pelteobagrus

Pelteobagrus belongs to Siluriofmres, Bagrida. As a kind of small and famous economic fish, Pelteobagrus is well accepted by consumers. At present, there are only five species in this genus, named Pelteobagrus fulvidraco、Pelteobagrus eupogon、 Pelteobagrus vachelli、Pelteobagrus nitidus and Pelteobagrus intermedius, only classified by traditional morphology. So there are still some arguments on the validity of these species. This article is about the research on the species classification of Pelteobagrus by using morphology and molecular biology methods to clarify the confusion and find other species of Pelteobagrus. It not only provided the evidence for the relevant departments to make the effective and rational breeding plans, but also to solve the problem of advantage varieties, fingerling breeding and production, which restrict the industrialization development of Pelteobagrus.A total of13morphometric measurements and17truss network datas were measured in4species of Pelteobagrus of Poyang Lake to evaluate the morphological variations and species validation by combination of multivariate morphometrics with traditional morphology. The results of clusters show that P.vachelli and P.eupogon were clustered for their minimal difference, then clustering with P.nitidus. Finally, P.fulvidraco was clearly found to be separated from others. The principal component analysis revealed that morphometric characters were coincident with the cluster analysis. Discriminant analysis finds that comprehensive identification rate of discriminant function is95.08%, it can get92.62%after cross-validation. The result showed by three kinds of multivariate analysis indicated that4species of Pelteobagrus are valid species.13pairs of SSR primers were used to study the classification of Pelteobagrus. The results showed that10of13SSR primers are valid. Amplified PCR product has a total of93polymorphic bands, the number of bands’ range is8-11, amplified fragment length is between90-400bp. The genetic similarity coefficient of5species strains a range of0.670to1.000. The results of clusters are same in SSR and morphology. P.vachelli、P.eupogon and P.intermedius were clustered for their minimal difference, then clustering with P.nitidus, P.fulvidraco was clearly found to be separated from others.46pairs of SRAP primers can amplify fragment,19of them can be used on research for its high polymorphic and distinct bands. Amplified PCR product has a total of179polymorphic bands, the number of bands’ range is5-15, amplified fragment length is between90-510bp. The genetic similarity coefficient of5species strains a range of0.670to1.000, the genetic differences between species was high in Pelteobagrus. The results of SRAP showed P.vachelli was separated from others, P.fulvidraco、P.eupogon and P.intermedius were clustered for their minimal difference.The results of molecular markers and morphologic method show that5species of Pelteobagrus are valid species. The discriminant function of4species can be used for the rapid identification of Pelteobagrus. Polymorphic bands from SSR and SRAP can be successfully used to classify Pelteobagrus and reflect the genetic differences between species. It is an important means for compensating the shortage of morphological classification. The result of genetic diversity showed Pelteobagrus germplasm resources were high and genetic diversity was rich. This will help more comprehensive analysis and theoretical studies on the relationship and systematic evolution of Pelteobagrus. ndexes of genetic diversity were close to or slightly higher than other populations which had reported. In addition, we found Pelteobagrus was miniaturization and in lower age. Attention should be paid to this by corresponding departments. Protection and measures should be done to keep the germplasm resources of Pelteobagrus.