Studies On Nutritional Value And Antimicrobial Activity Against Resistant Escherichia Coli Of29Kinds Of Chinese Herbal Medicines

In order to research new replacer of antibiotic, the study was conducted to analyzecontents of nutrients in29Chinese herbal medicines and determine their effects on in vitrofermentation characteristics by using Cecla digesta as the inoculums collected fromNingxiang pig feed nonomral diet. It was determined antimicrobial activity of the singleChinese herbs and its compounds against13isolated strains of drug-resistant E. Coli withdrilling a hole and double dilution method, and observed protective effect of the Chineseherbal for mice challenged by drug-resistant E. Coli. The results are as follows:(1) The contents of routine nutrients and amino acids. Contents of gross energy(21.33MJ/kg), ether extract(17.25％), crude protein(21.35％) in Semen allii Tuberosi were higer,as well as contents of Crude ash (26.23％) in Portulaca oleracea Linn, when comparedwith other herbal medicines. Furthermore, contents of amino acids, especially someessential amino acids are rich in these herbs, Semen allii Tuberosi has higher on contents of15kind of amino acids except for Met and Tyr, and especially of Glu and Arg as much as3.88%、2.34%.(2) In vitro fermentation characteristics of Chinese herbal medicines. The maximumvolume and rate of gas production in Radix scrophulariae, White atractylodes rhizome,Rhizoma anemarrhenaw, Gentiana scabra Bunge, Cortex dictamni and Crataeguspinnatifida Bun-supplemented groups were higher(P<0.05) than the free-supplementedgroup, while the lag time of gas production, pH value and NH3-N content of Radixscrophulariae and Crataegus pinnatifida Bun were lower than the other groups and thefree-supplemented group. Contents of acetic acid, propionic acid, butyric acid, isobutyricacid, valeric acid and isovaleric acid in the Radix scrophulariae, White atractylodesrhizome, Rhizoma anemarrhenaw, Gentiana scabra Bunge, Cortex dictamni and Crataeguspinnatifida Bun-supplemented groups were lower(P<0.05)than the the other groups and thefree-supplemented group.(3) Antibacterial activity of single Chinese herbal medicine extracts. Extracts fromChinese nut-gall, Crataegus pinnatifida Bun and Fructus forsythiae have activity to13tested E. Coli strains, ethanol extracts from Chinese nut-gall were higher than20mmagainst all of the tested E. Coli strains. The ethanol extract from Chinese nut-gall showedthe best activity, with MIC values of15.6mg/ml, and of its aqueous extracts was in therange of125-250mg/ml against13tested E. Coli strains.(4) Antimicrobial activity of chinese herbal compounds. compound Ⅴ(Consists ofChinese nut-gall, Crataegus pinnatifida Bun and Fructus forsythia) had the best activity to13tested E. Coli strains.(5) Protective effect of the Chinese herbal for mice challenged by drug-resistant E coli.Chinese herbl compoud can significantly improve in the length of duodenum villi, jejunumvilli, and protection ratio is50%after challenge.