Basic Study Of A New Biological Chinese Herbal Medicine, Bioprocessing And Its Application In Broiler

Three experiments were conducted for preparation of Biological Chinese herbal medicine and its application in broiler. Established the model of selecting raw materials and explored the fermentation parameters.1 .Researched synergistic effects between Chinese herbal medicine and Microorganism through measure OD by spectrophotometry (λ=660nm). The results show that the extractions of Radix Astragali, Radix codonopis had marked anxo-action to Bacillus 1.769, SOD, D01, Lactobacilli LB006, Rhodotorulasp, Yeast(P<0.01). But the extractions of Radix et Rhizoma Rhei, Rhizoma Woodwardiae had produced marked inhibition to them. Radix Astragali had marked anxo-action to LB006 in 0.0125% level (P<0.01), and improved biomass 62.6% in 0.1% level. So selected Radix Astragali and LB006 to prepared Microecological Chinese herbal medicine products.2.Many factors were explored which influence fermentation result. The optimum fermentation parameters are: concentration of substrate 5.0g/100ml, reaction time 36h, inoculum's concentration 0.5ml(9.867×10~8CFU/ml), disinfection temperature and time 121℃ 20min, rotational speed 140r/min. Concentration of LB006 is beyond 109 CFU/ml in zymotic fluid. Effect of substrate, reaction time, disinfection and rotational speed are significant(P<0.01), while effect of inoculum's concentration is not significant (P>0.05).3. Two hundred and fifty broilers of 1 day were randomly divided into 5 groups. Treatment group A was fed basal diet, group B was fed antibiotic diet, group C, D, E were fed basal diet fortified with Radix Astragali , Biological Chinese herbal medicine A and B. At the termination of feeding trial, researched the Performance of chickens, the change of immune function and intestinal microbiota. The results showed that during week 0 to 4: Biological Chinese herbal medicine A and B obviously increased broiler's average weight gain and average feed intake (P<0.01), while feed conversion rate showed no significant difference from other groups. They could improve the rate of conversion of T lymphocytes (P<0.01) compared with others, but its influence to the weight of thymus, spleen and bursa of Fabricius is not significant. They can promote the growth of probiotics (such as Lactobacillus) and reduce pathogenetic microorganism (as E.coli.) (P<0.01), adjust the balance of intestinal microbiota, protect body health. Biological Chinese herbal medicine produced better result compared with antibiotic diet at integrality.