| In order to investigate the effect of each constituents of the Chinese herbal medicine prescriptions in preventing the sub-clinical mastitis, we carried out a series of experiments using separate herbs of the conventional prescriptions in Black-White milking cows with CMT method. 48 milking cows of the same body weight , fetal sequence and the same duration of milking period were allocated to six groups ,which consisting of 8 cows. We had different management on every group. The Chinese herbal medicine in the 1st group were Semen Persicae and Flos Chatham, which had quickening the blood flow and removing stasis effects; The herbs used in the 2nd group were Semen Vicariate and Retrieves Fluffier Fructose, which can free the channels and quicken the network vessels. The 3rd group used Herbal Taraxaci, which had clearing internal heat and resolving toxin effect. Those in the 4th group were Radix Astragals and Herbal Epimedii, which had tonic (and boosting) effect. The 5* group was treated with a fine preparation of all the above medicinal herbs; Every group used medicines once a day during the term of 7days. The 6* group was kept as control group, without any medicine treatment. Milk samples were collected in every group on day 0, day 7,day 14,day 28,and day 60 for the CMT test , bacteria isolation and recording daily milk production. The results indicate that the positive rate of CMT, the number of bacteria and the milk production in the 1st, 2nd and control groups were not significantly different which showed Semen Persicae and Floss Chatham, Semen Vicariate and Retrieves Fluffier Fructose had no clinical effects on sub clinical mastitis. The positive rate of CMT in the 3rd group had no significant difference in statistics (p>0.05); The positive rate of CMT in the 4th group and control group had no significant difference (p>0.05) on day 14, but had significant difference (p<0.05) on day 28, but the significant difference was disappeared on day 60 .The positive rate of CMT36in the 5th group and control group had significant difference (p<0.05)on day 14, and had most significant difference (p<0.01) on day 28. Two kinds of bacteria桽taphylococcus and E.coli were isolated from the milk. The bacteria were used in this test. The number of bacteria in the 5th group was lower than that in the control group, and the difference was significant on day 14 (p<0.05). The milk production in the 3rd and 4th groups improved greatly 28 days post-management. The production in the 5th group improved mostly 14 days post-management, the difference was significant between the two groups (p<0.05). Above all, the medicine in the 5th group was the best method to treat sub clinical mastitis. We had the test of exoteric inhibited bacteria and some immunological tests in the 3rd, 4th and 5* groups. In the inhibited bacteria test, we set up the environment just like that within the body of the cow, and inoculate Staphylococcus and E.coli into defatted milk instead of meat soup. The results showed that the direct effect of the three groups were not strong. In immmunlogical test, we used Kun-Ming mice as experiment animals and used the medicine of the three groups separately for the mouse to survey the weight of thymus, spleen and the function of macrophages to engulf the chicken red blood cells. The result showed that the medicine in the three groups would increase the weight of thymus and spleen, and improve the function of macrophages to engulf the chicken red blood cells. The medicine in the 4th group would improve the index of spleen significantly (p<0.05) and that in the 3rd group would improve the function of macrophages to engulf the chicken red blood cells significantly.
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