Regulation Of Different Feed Additives On Inosinic Acid Deposited In Broiler’s Muscles And Its Enzyme Activity In Muscle

The paper was conducted to study the regulation of inosinic acid content in broiler’s muscles byfeed additives and its mechanism. Feed additive that can have a significant effect on AA broilers IMPcontent was screened out by two feeding experiments. Finally, the taste test provides proof for effects offeed additives in the experiment Ⅱ.Experiment14201-d-old male Arbor Acres broilers were randomly allocated into7dietarytreatments including control,0.3%nucleotide,0.01%L-carnitine,0.1%betaine,1%glutamine,0.1%tea polyphenols,23mg/kg soybean isoflavone. Birds were slaughtered at42days old. The resultsshowed that L-carnitine had a significant increase in average daily feed intake and average daily gain,compared with control group (P<0.05). There was a significant difference in abdominal fat percentagebetween the experiment and control group (P<0.05), except nucleotide and tea polyphenols group. IMPin breast muscle of broilers in groups of nucleotide, betaine, glutamine, tea polyphenols and soybeanisoflavone groups were significantly improved41.73%,33.42%,29.37%,31.95%and37.35%than thatin control group, respectively (P<0.05), with the exception of L-carnitine.In conclusion, supplementing feed additives of nucleotide, betaine, glutamine, tea polyphenols andsoybean isoflavone could drastically improve deposition of IMP of broilers in the circumstances ofnon-adverse effect on growth performance, carcass characteristics of broilers. Nucleotide, betaine andsoybean isoflavone were screened out when the price of feed additives and incresment rate of IMPdeposition were comprehensively considered.Experiment2A total of2561-d-old male Arbor Acres broilers were randomly allocated into8dietary treatments including control,0.3%nucleotide (N),0.1%betaine (B),23mg/kg soybeanisoflavone (S),0.3%nucleotide+0.1%betaine (NB),0.3%nucleotide+23mg/kg soybean isoflavone(NS),0.1%betaine+23mg/kg soybean isoflavone (BS),0.3%nucleotide+0.1%betaine+23mg/kgsoybean isoflavone (NBS). Birds were slaughtered at42days old. The results showed that there was nosignificant difference in performance traits and carcass characteristics between any trial group andcontrol group (P>0.05), except higher feed conversion ratio in NS and NBS groups and lowerabdominal fat percentage in B, S, BS and NBS groups (P<0.05). Depositions of IMP in broiler’s breastmuscle in N, B, S, NB, NS, BS, and NBS groups were significantly improved41.33%,34.67%,37.33%,41.33%,26.67%,38.67%, and42.67%than that in control group, respectively (P<0.05). No differencewas noted among test groups (P>0.05). Broilers in group N had a lower activity of5’-NT than that incontrol group (P<0.05). Significantly higher ATPase activity and lower ALP activity in the breastmuscle of birds were found in B group than that in control group (P<0.05). Soybean isoflavoneincreased ADA activity and decreased ALP activity significantly (P<0.05). All combination groups hada higher activity of CK and ADA than that of the control group (P<0.05), except group BS and NBS.ALP activity of group NS was higher than the control (P<0.05).In summary, supplementing feed additives of nucleotide, betaine, soybean isoflavone and their combinations could drastically improve deposition of IMP of broilers in the circumstances ofnon-adverse effect on growth performance, carcass characteristics of broilers by increasing activity ofsynthase and inhibiting activity of degrading enzymes, of those, the greatest incresment of IMP wasshowed in NBS supplemention group, followed by N and NB.Experiment3This experiment was conducted to test the datas from experiment2. The resultsshowed that these additives and their combinations improved the meat quality in color, aroma,tenderness, juiceness and fresh in some degree. Therefore, they can be applied to production practice.