AMF On Cool-season Grasses Function And Improve The Tolerance Capacity To Low Temperature Stress

Turfgrass play a significant role in the processes of soil and water conservation,as well as the retention and purification of non-point source pollution. In the northcold area, low temperature stress has a real impact of a different degree on theestablishment, growth and physiological activity of Turfgrass, especially in earlyspring when spring flood hits in concentrated form and Turfgrass vegetation srevive and establish slowly. Consequently, it will bring serious non-point sourcepollution in river basin. Therefore, what we need urgently are choosing excellentcool season grass species and improving their performances in growth, resourceutilization efficiency and stress tolerance by artificial enhanced techniques, aimingto guarantee their prompt establishment and ecological barrier function at lowtemperature in early spring.In the present research, based on the excellent properties of arbuscularmycorrhizal fungi (AMF) in plant growth, resource utilization efficiency and stresstolerance, the indigenous good AMF, Glomus mosseae (GM) and Glomusintraradices, were selected as the experimental inoculum to establish the AMF-poapratensis barlin symbiosis system, and then to investigate the improved impacts ofsingle inoculum and mixed inoculum (MI) on the growth, carbon resourceutilization efficiency, water utilization efficiency, low temperature stress toleranceand retention efficiency of total N and total P of poa pratensis barlin, etc.Based on the analysis of results, we found that all the AMF inoculation cansignificantly (p<0.01) improve the infection of AMF to poa pratensis barlin, MI hasthe highest infection rate (54.31±0.31%) and the strongest mycorrhizal dependence(128.06%). AMF inoculation can increase the plant height, leaf area, root shootratio, and underground biomass, meanwhile, decrease the stem leaf ra tio. Comparedto CK, MI increased the aboveground and underground biomass (24.46%and59.62%, respectively). AMF inoculation effectively increased the utilizationeffic iency of total N (6.0%) and total P. AMF inoculation enhanced the Pn and WUEof poa pratensis barlin. At the low temperature, AMF inoculation increased thecontent of chlorophyll in the leaves of Poa Annua and the activities of SOD, CAT,etc. Simultaneously, AMF inoculation decreased the relative permeability ofplasmalemma and the content of MDA, which enhanced the tolerance capacity tolow temperature stress.The main conclus ion was: the mixed inoculum(MI)showed the highestinfection rate, strongest mycorrhizal dependence, and the most stable symbioticrelationship. And, MI could efficiently relieve the low temperature stress resultedfrom the short sun shining time in early spring, and simultaneously, improve the capabilities of poa pratensis barlin in establishment, stress tolerance and retentionof non-point source pollution. Therefore, MI is a suitable efficient organisminoculum for buffer zone vegetation, and expected to be applied as a safe, efficient,economical, pollution-free, and new method to improve the establishment of bufferzone vegetation in early spring and enhance their function.