Screening And Functional Study Of Novel Biomimetic Antibacterials

Using biomimetic synthesis technology, we obtained a novel antimicrobial agentα-propargyl-γ-methylene-γ-butyrolactone on the basis of protoanemonin. Wecharacterized bacteriostatic effect and antimicrobial mechanism of the agent usingphysical and chemical analysis. We carried out the minimum inhibitory concentration(MIC) determination, suspension quantitative germicidal test, and other tests to verifyits antimicrobial effect, the mechanism of which were primarily studied through cellwall integrity test and transmission electron microscopy. We found that the novel agentα-propargyl-γ-methylene-γ-butyrolactone had a good inhibitory effect on variousbacterial species, especially on Malassezia globosa. Its MIC to malassezia was0.5mg/ml. Our suspension quantitative germicidal test, using Sodium thiosulfate asneutralizer, showed a high antibacterial efficiency that up to99.99%of Malasseziaglobosa is killed after exposed to10mg/ml agent for only1min. Membrane integrityexperiments and transmission electron experiment suggested that the antimicrobialeffect is not due to destroying the cell wall systerm, but it may have some impact on thecell wall system.