Research Of Plant Tissue Culture Technology Of Plumbago Auriculata

The main propagation method of Plumbago auriculata is seed sowing. Due to low fruiting rate, the seed price is expensive. Plant tissue culture is widely used plant micropropagation method to produce plant seedlings and it can offer certain advan-tages over traditional methods of propagation. So plant tissue culture may be used to enhance breeding rate and large-scale production of Plumbago auriculata.With the explants of young nodals and leaves of Plumbago auriculata, we stu-died explants selection, sterile technique of the explants, multiple shoots induction and plant regeneration in the medium with different PGR proportions. The results of the experiments are as follow:1. Compared with leaves explants nodals of Plumbago auriculata could be used as the best explant to induce multiple shoots.2. MS medium is better than WPM medium in plant tissue culture of Plumbago auriculata.3. The best sterile technique of the explants was surface-sterilized nodals in75.0%alcohol for45s and in0.1%mercuric chloride solution for10min.4. Results showed that the effects of different concentrations and combinations of PGR on induction of single shoots were different. Induction rate of single shoot dropped significantly with increasing concentration of2,4-D. Mean analysis showed that the best medium for single shoot induction was MS plus0.4mg/L6-BA, which was consistent with the testing result.5. The effect of different concentrations and combinations of PGR on the induc-tion of multiple shoots was different and range analysis showed that6-BA played the most important role in the multiple shoot induction. Mean analysis showed that the best medium for multiple shoots induction was MS+1.0mg/L6-BA+0.3mg/L NAA+0.5mg/L IAA, which was consistent with the testing result.6. The percentage of shoots forming roots and the time began to root significant-ly varied with the concentrations of IBA and NAA and the optimal rooting and growth of single shoots were observed on medium containing0.3mg/L NAA. At higher concentrations of IBAand NAA, rooting was inhibited but callusing occurred at the cut ends and the rate of root induction was only31.67%.7.2,4-D plays an important role in induction of callus. MS+1.0mg/L6-BA+1.0mg/L NAA+0.06mg/L IAA+1.0mg/L2,4-D was the best medium to induce callus. But the callus stopped growing in the end.