Plant Regeneration From Shoot Tips In Triploid Loquat

In the present study, plant regeneration was completed for the first time using shoot tips as explants harvested from a triploid mother plant in loquat (Eriobotrya japonica). A series of experiments were carried out to investigate the effects of disinfection reagents and disinfection time, time of explant isolation, size of explants, growth regulators on contamination, browning, shoot tip sprouting, elongation, multiplication, rooting and transplantation. The main results are as follows.1. The results showed that an optimal disinfection result could be obtained in 75% alcohol for 12s+0.1% mercuric chloride for 8min, resulting in a comparatively low contamination rate (12.6%) and browning rate (20.3%) as well as high survival rate (67.1%).2. Explant isolation in the Spring and shoot-tip size of 0.7 cm were appropriate, resulting in a survival rate of 77.5%.3. The appropriate initial culture medium was MS+6-BA 1.0 mg · L-1+NAA 0.5 mg · L-1+NAA1.5 mg · L-1, in which the sprouting rate and average shoot length reached 91% and 2cm, respectively, with good shoot growth status,4. The suitable medium for shoot elongation was MS+6-BA0.5 mg · L-1+NAA0.3 mg · L-2+GA30.3 mg · L -1, which resulted in an average shoot height of 4.9cm.5. The most suitable medium for shoot proliferation was MS+6-BA1.0 mg · L-1+ NAA0.3 mg · L-1+Tryptone 750 mg · L-1, in which a high multiplication coefficient of 7.7 was obtained.6. The appropriate rooting medium was 1/2MS+NAA 0.25 mg · L -1+IB A2.0 mg · L-1, in which the rooting rate was 73.3%, average root number was 8.1 and average root length was 4.2cm with good plantlet growth status.7. The rooted plantlets, after hardening, survived at a rate of 93.67% with well developed and deep green leaves, thick shoots and good plant growth status in the transplantation matrix of decomposed organic fertilizer, garden soil and sawdust(1:2:1) under careful temperature and water management.