Analysis And Evaluation Of Functional Components In Different Varieties Of Sweet Potato Stem And Leaf

In this paper, using high performance liquid chromatography(HPLC), for the first time on a varietyof sweet potato leaf extracts were analyzed by fingerprinting construction, provides a method for theidentification and classification of sweet potato varieties. At the same time through the forint phenolcolorimetric method and sodium nitrite-is determined by aluminum nitrate method39differentvarieties of sweet potato leaf, the content of total flavonoids and total polyphenols in and through HPLCchromatography determination of chlorogenic acid in the stems and leaves, the content of caffeic acidand ferulic acid for the development and utilization of sweet potato stem leaf resources, provides thequantitative detection and analysis method.Test results show that the total flavonoids and total polyphenol content of sweet potato leaves wereobviously higher than that of stem, stem leaf between different varieties of total polyphenols andflavonoids content is a significant difference, the content of total polyphenols and flavonoids have acertain positive correlation. The test samples, and higher levels of total polyphenols and flavonoids ofseveral varieties of chongqing respectively18, zhejiang in132and263, xu potato Su Ru303.Sunfire by RP-HPLC, using C18ODS chromatography column, acetonitrile and0.5%acetic acidaqueous solution as mobile phase and in30℃under the condition of column temperature and detectionwavelength of325nm, establish stem leaf of chlorogenic acid, caffeic acid and ferulic acid contentdetermination method of effective ingredient content of differences between different varieties. Resultsshow that this law USES the gradient elution can be finished within30min in chromatographic analysisof component, and has good isolation between the chromatographic peak of each component, thetheoretical plate number is3000; Chlorogenic acid, caffeic acid and ferulic acid in a linear relationshipwithin a certain concentration range, the method is simple, sensitive, rapid, accurate, reproducible andcan be used for the sweet potato stem foliage and preparation of quality control and evaluation.Sweet potato leaves in the fingerprint study is less, this paper chose13total polyphenol andchlorogenic acid content is higher of sweet potato varieties, with chlorogenic acid as reference, toestablish the HPLC fingerprint of sweet potato leaves, provide the basis for identification and qualitycontrol of sweet potato leaves. Method to determine the total of12for fingerprint chromatogram peak,by similarity analysis and evaluation, found that the higher similarity between the sample spectra.