| The Liaoning new-breeding cashmere goat is a unique genetic resource in china. It isfamous for cashmere yield and good quality. Cashmere is the inner layer of the goat hair,which is the outcome of secondary hair follicles and its fiber with light, slippery, soft, warm,and other characteristics. The excellent quality of wool has a great relationship with keratinand keratin-associated protein. The keratin form the skeleton of the hair, which has a contentand structure that are relatively stable. But the content and structure of KAPs differs greatly indifferent species’ hair. So KAPs may play a major role in the quality of hair. In order to studythe relationship of KAP family gene expression with cashmere fineness, firstly bioinformaticsanalysis was used for KAP26.1and KAP11.1gene. Semi-quantitative RT–PCR was used todetect the expression of the KAP26.1and KAP11.1gene in all internal organs. Real-timePCR was used to discover the expression levels of KAP11.1gene in the primary hair folliclesand secondary hair follicles during anagen, catagen, and telogen.Finally,we used in situhybridization to detect expression position of KAP26.1and KAP11.1gene in the primary andsecondary hair follicles.(1) A bioinformatics analysis showed that KAP11.1gene encodes a putative159amino acidprotein that exhibited a high content of cysteine, serine, threonine, and valine and has a PMGstructural domain. The KAP26.1protein included184amino acids, and showed a relativelylow aspartic, methionine, cysteine, tryptophane content, but a high amount of serine andleucine, and had a serine-rich region. In addition, KAP26.1protein possessed severalcarboxyterminal tyrosine-containing tandem decameric repeat structures.(2) Semi-quantitative RT-PCR results showed that KAP11.1was expressed in skin, heart, andliver, but not expressed in lung, spleen, and kidney. KAP26.1was only present in cashmeregoat skin.(3) Real time PCR illustrated that the levels of KAP11.1expression in the secondary hairfollicles was2.5times more than in primary follicles during anagen; however, during catagenthe expression of KAP11.1in the primary hair follicles was2times more than in secondaryfollicles.And the levels of KAP26.1expression in the secondary hair follicles was4.74timesmore than in primary follicles during anagen; during catagen the expression of KAP26.1inthe primary hair follicles was3.84times more than in secondary follicles.(4) In situ hybridization analysis showed that KAP11.1had a signal in the inner root sheath. Additionally, a clear signal was found in matrix cell as well. The hair bulb had a smallamount of expression. KAP26.1was specifically expressed in the inner root sheath. Thedifferent position of KAP11.1and KAP26.1gene in Liaoning new breed of cashmere goatskin might lead to the differences of wool quality. |
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