Expression Analysis Of POMP Gene In Hair Follicle Development Of Liaoning Cashmere Goat

We have acquired the total length of the sequence of the POMP(proteasome maturation protein) gene of Liaoning cashmere goats by constructing a skin c DNA Library. To explore the relationship between POMP and cashmere growth, We analyzed the c DNA and amino acid sequences by biological informatics analysis, and established evolutionary tree. Then we analyzed the structural domain and hydrophobicity, and forecasted secondary structure, transmembrane domain, phosphorylation site and signal peptide. Semi-quantitative RT–PCR was used to analyze the expression of the POMP gene in all internal organs. Real time-PCR was used to detect the contents of POMP gene expression in the primary and secondary hair follicles during different growth periods. The POMP gene was detected specificity expression sites by In situ hybirdization. Otherwise, Fibroblasts were treated with melatonin,FGF5 and IGF-1 respectively for different hours to explore the optimum of treatment duration and concentrations, in order to further exploration of the mechanism between melatonin, IGF-1 and POMP. In addition, Fibroblasts were treated with melatonin and FGF5(melatonin and IGF-1) synergeticly, to explore the synergetic manner between them.The results of biological informatics analysis were as follow: the results of POMP gene showed that it could encode the protein including 141 amino acids. It`s full length is 1278 bp. Randon coil is the main component of Secondary structure of POMP. Prot Scale showed that proteasome maturation protein is hydrophilic. Function site analysis showed that POMP doesn’t have a transmembrane domain, or signal peptide, both demonstrated that the encoding protein of POMP gene is most likely in cytoplasmic matrix or organelles matrix. There are 4 serine phosphorylation sites. RT-PCR detect showed that POMP were expressed in skin, heart, liver, spleen, lung, and kidney tissues. Real-time PCR results showed that: the expression was more active in the secondary hair follicles than that in the primary hair follicles in both anagen and catagen. In situ hybirdization showed that POMP was obviously expressed in the Inner Root Sheath(IRS) but no expression in Outer Root. The treatment of fibroblasts with melatonin and IGF-1 showed that: expression of POMP is higher than the control group, which means that the synergy of melatonin and IGF-1 can improve the expression of POMP.