Muskmelon Fruit Tissue Structure Observation And Construction Of SSH Library Of Muskmelon After Pollination

We have used the Muskmelon (Cucumis melo L.) M01-3as the test material, the totalRNA were extracted from the fruit before and after pollination, and used suppressionsubtractive hybridization technology to construct the muskmelon fruit fruit at different growthstages to find the related gene subtractive cDNA library.48of sequence were obtained by notrepeated EST; at the same time we observed the structure of muskmelon fruit in development,discussed the physiological changes during the fruit in the development.1. Observed the histological structure of fruit development of melonWe observed the histological structure of fruit development of melon by paraffin sectionmethod．The results indicated that the exocarp of melon was composed of epidermis，epidermis hair and stoma．The cleavage of the exocarp cells is mainly in vertical direction tomake fruit expand during young fruit growth．With fruit development，there are somesubstances containing xylogen filled in the spaces of epidermis cells and there is a quite thickcuticle layer formed on the surface of epidermis．Melon mesocarp can be divided into outermesocarp including parenchyma cells and elongation cells and inner mesocarp composed ofspongy tissue and vascular bundle．Mesocarp cells arrange close and the size and appearanceof all cells have no big differences at the young fruit stage．The cleavage of mesocarp cellbecomes slow and the cell volume is larger and larger as the fruit development．During fruitmature，the cell walls of some cells in the inner mesocarp break up and cells inoculate eachother．The cellulose，a component of cell wall begins to dissolve，and finally，the fruitbecomes soft and mature.2. subtractive library constructionMuskmelon of ‘M01-3’ was used materials to construct the library onpollination-induced subtractive, we found some sequences was related to photosynthesis,protein synthesis, cell structure, signal transduction, transcription, metabolism. There are42EST sequences induced reaction related. Results showed that:7sequences of protein synthesisgenes,5sequences of signal transduction related gene,1apart of proteinmodification/processing/storage, cell division gene has2kinds, there are6types of cellstructure genes, transcription factors are6,9sequences of metabolism. There were not classified on5genes. At the same time, the results also found that, Those EST to participate inthe metabolism. And other functions related to protein synthesis and gene accounted for alarger proportion of.3. The metabolism and primary carbon metabolism genesWe found a cucumber with vacuolar ATPase subunit E was highly homologous to thesequence of EST (No.63), the consistency is achieved94%, predict the possible developmentof muskmelon pollination and start. A cucumber malate--aspartate shuttle was highlyhomologous to the sequence of EST (No.81), the gene can accelerate the three carboxylicacid cycle process, produce more organic acid secretion to the apoplast in muskmelon fruit,thus speeding up the fruit of the new supersedes the old.4. transcription and signal transduction genesThe study also found a (02) polyadenylate-binding protein [poly (A)-binding protein,PABP], PABP is a kind of proteins can with mRNA3′Poly (A) combined with highlyconserved, it was involved with mRNA translation and regulate its stability qualitative. Wefound in the library of (119)、(191) and (14) genes involved in calcium signal transductionand control the protein kinase fruit after pollination, have important roles in pollengermination and growth of young fruit primary.5. The cell structure associated genesResults showed that,(22) EST sequences and cucumber tubulin gene are so similarity ofhigh up to83%, E value:5e-47. The gene may be after pollination and fruit fruit auxintransport has great relevance. In this study we also found a molecule of water channel proteinTIP1(No.36), the regulation of water molecules in the vacuole membrane import, that youngfruit by pollination induced expression of water channel protein to relieve the environmentalosmotic pressure.