In Vitro Culture And Plants Regeneration Of Flos Lonicerae Xianglei

Flos Lonicerae Xianglei from the series of varieties of Lonicera macranth oides natural mutation breeding in1997. Especially the Lonicera macranthoides ’Huawang’has always into flower bud, neat, green, densely, much branched, tree-like developed, florescence is long, the content of chlorogenic acid is high er in Hunan, Xupu and Longhui are particularly popular. The propagation coeff icient and the propagation speed of cutting and grafting is limited, and the exi sting of Lonicera macranthoides in vitro tissue culture technology proliferation coefficient is low, it is difficult to meet the market demand for the quality of seedling.Lonicera macranthoides’ Huawang’is excellent comprehensive characters, high content of chlorogenic acid. In vitro culture and plantlet regeneration were studied of stems, petioles, leaf as explants, in order to lay the foundation about study of Flos Lonicerae Xianglei germplasm innovation, variety improvement and genetic transformation.The following are the main results:1. The better method of sterilization is selection of the annual shoot growth season, cold treatment after7days,15-20s with75%alcohol, then12min with0.1%HgCl, cultured for20days,the contamination rate was13.3%, the survival rate was76.7%,8.9%higher than the1%hypochlorite treatment.2. The formula for the induction rate higher than other medium of callus culture medium in MS+NAAl.Omg/L. And petioles as explants induction rate was81.3%,the highest; followed by leaf, stem segments of the induction rate was76.9%; the worst, the induction rate was68.4%.3.A suitable for Callus differentiation formula for MS+6-BA1.5mg/L+NAA0.1mg/L, after30d culture, the differentiation rate was75.6%. Adventitious bud increment formula is more suitable for MS+6-BA1.Omg/L medium, after20d culture, value-added ratio reached6times.4. A suitable rooting formula is l/2MS+IBA0.2mg/L+NAA0.2mg/L, after40d culture, rooting rate was76.7%. The tissue culturing seedling in seedling perlite in15days, the survival rate was higher,75.5%, rate of up to100%survival after transplanting. 5. Through the different light quality on callus induction and proliferation and differentiation, not bud and rooting, found in the white light effect is better than the dark, red and blue light. White light than dark, blue light and red light, stem and petiole callus induction rate of average high of4%,17.8%,25.5%, the callus differentiation rate respectively24.4%,12.2%,17.8%, value-added ratio adventitious bud were increased6times,3times,3times, the rooting rate increased by37.8%,5.6%,4.5%.6.Determination of Lonicera macranthoides’Huawang’ tissue culture ma-terial, seedlings and the female of the content of chlorogenic acid content. Ch-lorogenic acid content was the highest tender callus, the content of chlorogenic acid was8.3%; followed by tissue culture the seedling transplanting, chlorogenic acid content was7.6%. The new content of chlorogenic acid in callus and seedlings in the female than in old leaves were increased by0.9%and0.2%.