The Tissue Culture And Rapid Propagation And Callus Induction Of Photinia Frasery Parvifolia

Photinia frasery’Parvifolia’is a evergreen shrub which belongs to rosaceae. It is a dwarf and leaflet variety of Photinia frasery. Compared with traditional Photinia frasery, it is superior line which had longer red-leaf period, better frost resisting ability, stronger tolerance to barren soil, and stronger sprout capacity. The initial explants were tender stem segments with axillary buds, the tissue culture and rapid propagation system of Photinia frasery was established in this paper based on the researches of axillary buds induction, shoot proliferation, root induction and acclimatization and transplants. Systematic research were also conducted on the optimum hormone combinations for callus induction from leaves, petioles. The main results are as follows:(1) The tender branches were collected from march to April as explant. Using0.1%HgCl2to set up different time (5min,8min,11min)and2%NaClO with (15min,20min,25min,30min) for disinfection trials. The suitable disinfection method is0.1mg/L HgCl2with8min, the germination rate can reach to63.3%.(2) The suitable initial medium of stem segments with axillary buds was MS supplemented with6-BA2.0mg/L and NAA0.1mg/L, the Shoot initiation rate is96.7%, and the average shoot length is1.3cm.(3) Proliferation multiple of5.43and valid shoot ratio of87.3%and the average shoot length is1.63cm could be obtained by cultured the shoots in MS supplemented with6-BA2.0mg/L and IBA0.2mg/L and KT0.5mg/L.(4) The suitable rooting medium is1/2MS supplemented with sugar20g/l and IBA0.5mg/L and NAA0.1mg/L, the root induction is93.3%, average valid shoot strip is5.7, and average root length is4.17cm.(5) Appropriate matrix was peat:husk ashes:sawdust:pearlite=6:2:1:1, the transplanting survival rate could be93.3%.(6) In the research of callus induction, the callus induction rate from petioles is higher than leaves. The most appropriate medium for callus induction from petioles is MS supplemented with6-BA3.0mg/L and NAA0.2mg/L and dark culture for17days. The most appropriate medium for calli induction from leaves is MS supplemented with6-BA3.0mg/L and NAA0.3mg/L and dark culture for7days.