| The incidence of prostate diseases are very high in canine and prostatitis is one of these diseases frequently been seen in clinic. B-ultrasonic is an emerging discipline and developed very rapidly in recent years. B-ultrasonic could clearly display the thevarious sections of the organ and surrounding. Because of the feeling of substance and closer to the true anatomical structure, Early diagnosis via B-ultrasonic became an indispensable method in modern clinical medicine. However, the B-ultrasonic characteristics of canine prostatitis has not been reported. In this research, the artificial canine prostatitic model was established and the B-ultrasonic characteristics of canine prostatitis was analysed, to provide a theoretical basis and practical experience for the B ultrasound diagnosis of canine prostatitis. The main research content is divided into the following several parts:Part1:Preparation of pathogenic bacterium. Purpose:Preparation of effective inflammatory factor. Method:Separated clinical pathogens which could induce pericarditis, perihepatitis and peritonitis. The pathogen was identified on biochemical tests and PCR amplification of16SrRNA sequences of the bacteria and compared with E.coli. At the meantime, Drug sensitivity test and acute toxicity test were carry out. Results:The pathogen has the same biochemical characteristics with E.coli and when compared with the pathogenic E.coli reported in Gene Bank, high homology (99%) was found, so The pathogen was identified as E.coli. Drug sensitivity test showed that the bacteria was hypersensitive to Rocephin, Amikacin, Spectinomycin; moderate sensitive to Cefradine; hyposensitive to Nitrofurantoin, Gentamicin. Acute toxicity experiment found that all the infected mice were dead in48hours and the mice in control group were all health. The Escherichia coli was isolated in the infected mice. Conclusion:The Escherichia coli isolated was an effective proinflammatory cytokines.Part2:Establishment of a canine prostatitic model. Purpose:To establish an artificial canine prostatitic model. Method:Selected6healthy dogs and divided into two groups, control group (ldog) and Experimental group (5dogs labeled A, B, C, D and E). The fresh Escherichia coli isolated was washed and diluted10times with sterilized saline. Then the experimental dogs were injected with the diluted Escherichia coli into two leaves of prostate (2mL each leave) via surgical operation and the control dog was injected equal sterilized saline. After the surgical operation, the clinical symptoms were observed and the routine detection of blood and urine were carried out. Results:The body temperature after operation in experimental dogs were all increased.The clinical symptoms including depression, loss of appetite, vomiting, frequent urination, hematuria were observed. Due to pain, back arch was observed when urinary. The difference of hemoglobin (HB), red blood cell (RBC), and hematocrit (PCV) was obvious before and after surgical operation. Conclusion:Successfully established an artificial canine prostatitic model.Part3:To explore the value of B-ultrasound on the evaluation of the canine prostatitis. Methods:Using B-ultrasound to detect these artificial canine prostatitic models. Results: the difference of experimental canine prostate size is not obvious. Conclusion:the characteristic change of canine prostatitis detected by B-ultrasound is diffuse echo attenuate.Conclusion:(1) Use escherichia coli as proinflammatory cytokines, successfully established an artificial canine prostatitic model.(2) The characteristic change of canine prostatitis detected by B-ultrasound is diffuse echo attenuate. |
