| Continuous cropping cultivation is the important factor of the soil health deterioration and thedisease aggravation. Biological control, as it is amicable to the environment and can truly realize thesustainable development, has become the important way to solve the continuous cropping obstacles. Butas biocontrol microorganism is hard to colonize in the soil and become the dominant population, thenthe effect of biological is not stable, but the organic additives can be used as a carrier, helping biocontrolmicroorganism to colonize in the soil and improve the control effect.This experiment mainly studies the effect on plant root-knot nematode disease, plant growth, thesoil microbial diversity, the soil enzyme activity and the soil nutrient of organic additives cooperate withbiocontrol microorganism. Firstly we analyse several organic additives. Base on that, the optimalorganic material have been obtained. And further to study the effect of organic material combinationcooperate with biocontrol microorganism, and then it has been validated in the field of continuouscropping soil. The main results were as follows:1. Preliminary the evaluation result showed that cow muck, chicken manure and corn straw cansignificantly improve the plant’s underground fresh weight respectively by65%,106%and20.8%higher than that of control, aboveground dry weight respectively by120%,148%and94.4%higher thanthat of control. Chicken manure individual can significantly increase the bacteria in the soil, by401%higher than that of control, Cow dung individual treatment can significantly increase the actinomycetesin the soil, by297%higher than that of control. Cow muck, chicken manure could significantly increasethe stem diameter of plant, by29.5%and40.7%respectively compared with the control. The differenceof the effect on soil urease and alkaline phosphatase with the cow muck, chicken manure and corn strawwas not significant. The cow muck, chicken manure and corn straw can significantly increase the plant’sroot-knot index, by99.8%,30.2%and50.3%respectively higher than that of control.2. The results with organic matter influencing survival of biocontrol microorganism experimentshowed that the Bacillus licheniformis and Bacillus subtilis survival rate is highest to be10%in the cowdung under the condition of15%moisture content. Among them, the survival rate of B. licheniformis incow dung is0.77%higher than that of control. The survival rate of B. subtilis in cow dung is close to1.5%lower than that of control. The survival rate of Gliocladium roseum is highest to be10%in theCorn straw, by5.1%lower than that of control. The highest survival rate of Purpureocillium lilacinum is10%in the chicken manure, by93.9%higher than that of control. Under the condition of10%moisturecontent, the survival rate of B.subtilis in cow dung is6%higher than that of control, the same survivalrate of B. subtilis in chicken manure is only7.4%lower than that of control.3. The results of organic matter cooperating with biocontrol microorganism evaluation experimentcarried out in the pot showed that the P. lilacinum cooperate with organic additives exhibited asignificant control effect, and B. subtilis only with chicken manure or corn straw showed higher controleffect, but showed no significant control effect with cow dung. The control effect on the plant root-knot nematodes of chicken manure synergy with P. lilacinum is78.3%. The control effect of corn strawsynergy wih P. lilacinum is61.3%. The control effect of cow dung synergy with P. lilacinum is59.7%.The control effect of corn straw synergy with B.subtilis is70%. The control effect of chicken manuresynergy with B.subtilis is61.2%. Cow dung synergy with B.subtilis treatment showed no significantdifference. Corn straw synergy with biocontrol microorganism can improve the soil microbial diversity,and can be taken to reduce the number of root-knot nematodes, showing the great potential power ofbiocontrol. For example, it can significantly increase the number of bacteria, fungi and actinomycetes inthe soil, respectively by99.3%,82.4%and470.9%higher than that of control. Corn straw synergy withB.subtilis can significantly increase the number of bacteria, fungi and actinomycetes in the soil, by96.1%,158.2%and117.89%higher than that of the control respectively. Corn straw synergy withP.lilacinum and corn straw synergy with B.subtilis can significantly reduce the number of the soilnematodes, decrease respectively by63.59%and67.48%compared with the blank control. Chickenmanure and cow dung synergy with biocontrol microorganism can significantly promote plant growth,whereas corn straw performance is not obvious.4. Organic combination additive bioassy results showed that the additive1(P1) and the additive5（P5）significantly increase the actinomycetes in the soil, respectively by194%and58.28%higher thanthat of control. P1and P5both increased the sucrase of soil, respectively by35.7%and38.9%than thatof control. The control effect of the additive1(P1) synergy with B.subtilis (P1B) on the plant root-knotnematode is23.8%. Other treatments also showed control effect, The additive5（P5）synergy with P.lilacinum (P5P) exhibited the most significant effect of preventing and controlling root-knot nematodes,P5P decrease the plant root-knot nematode by60%. P1B significantly reduced the fungi in the soil, by21.94%lower than that of control, significantly increased the actinomycetes in the soil, by75.08%higher than that of control. P5P significantly increased the actinomycetes in the soil, by41.51%higherthan that of control. Effect of P1B on B.subtilis quantity in the soil was not significant. P5P significantlyincreased the P.lilacinum in the soil, by270.45%higher than that of control. P1B significantly increasedplant height, stem diameter, underground fresh weight and aboveground fresh weight, respectively by55.85%,48.45%,79%and48.45%higher than that of control; P5P has no significant affect on the plantbiomass. P1B significantly improved the activity of the soil sucrase, by61.45%higher than that ofcontrol. P5P significantly increased the soil sucrase activity, by80.31%higher than that of control. P5Psignificantly increase the content of available potassium in the soil, by5.25%higher than that ofcontrol.5. The results to the impact of efficient organic combination additive on the bacteria survivalexperiment showed that the highest levels of B.subtilis quantity appeared in P1to30days or so underthe condition of different water content. In conclusion, the test results indicated that15%of P1storageis ideal moisture content to the B. subtilis. To15days or so, the number of P. lilacinum in P5is to thepeak as1.57×107cfu g-1.6. The results of evaluation experiment that carried out in the field using efficient organiccombination additive cooperate with biocontrol microorganism showed that P5P and P1B exhibited a significant control effect on the tomato root-knot nematodes, through the50d after transplanting, thearea under disease development curve results showed that the area under disease development curve(AUDPC) value of P5P、P1B and the control was957.2、1937.1and957.2respectively, exhibitedsignificant difference (p <0.05). In the middle and later periods of the plant growth, P5P and P1Bsignificantly improved the aboveground fresh weight and underground fresh weight of plant, promotedplant flowering and fruiting ealier, significantly improve plants blossom and bear fruit, and improve theyield of plant by7.7%and20.5%respectively. P5P can effectively reduce the number of root-knotnematodes in soil, reduce by38.1%to the highest. The results of detecting P. lilacinum in the fieldshowed that P5P can significantly improve the biocontrol microorganism content in the soil, by49.8%higher than that of P. lilacinum alone, so we can know that suitable organic matter can promotecolonizing of biocontrol microorganism in the soil.The research results showed that organic additive cooperate with biocontrol microorganismtreatment can effectively inhibit plant root-knot nematodes infestation，reduce the plant root-knot index.It also can increase the soil microbial diversity and promote biocontrol microorganism colonizing in thesoil, inhibiting pathogens in the soil at the same time，improving the soil fertility and the soil physicalproperties, also promoting crop growth, increasing production of vegetables. From what has beendiscussed above, organic additive cooperate with biocontrol microorganism treatment can prevent andcontrol plant root-knot nematode disease，it is of great significance to the new preparation technique andapplied technology. |
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