The Study On Different Expression And Relationship Of BRCA1、VEGF And MVD In Sporadic Breast Cancer In Between Han And Mongolia Women

Background and Objective:Breast cancer is one of the malignant tumors which have a high prevalence andmortality rate in females through out the world, among which its prevalence andmortality rate in China have increased in resent years. it has become a threat to women’slives first death of cancer in multiple cities in our country, and The incidence of breastcancer in women of all nationalities is also increasing in Inner Mongolia, Mongolia is thedominant ethnic group in Inner Mongolia, they has a different genetic background andthe way of life than the Han Chinese, in Current the study of Mongolian women withbreast cancer is rarely in China. Two types of breast cancer can be divided into hereditaryand sporadic type, genetic-about5%, Most of them(95%)were sporadic breast cancer.Breast cancer susceptibility gene1(BRCA1) was hereditary breast and ovarian cancersusceptibility genes. Study finds, it is also a tumor suppressor gene, In1994, for the firsttime BRCA1was succeedly positioned, separationed and cloneding by Miki, afterScholars at home and abroad is having a lot of research on it, but most of them focus onthe family of hereditary breast cancer research, in sporadic breast cancer (SPC) there arerelatively few studies, Foreign research shows, BRCA1mutation show the differences incertain amount of regional and ethnic. And in Western European BRCA1mutation rate ofhereditary breast cancer in the people Ashkenazi Jewish was particularly notable, it is4times than other ethnic. there are relatively few studies on national and regional in China,Only, the differences between Uighur, Hui, Korean-Chinese and Han nationality wasstudied in Xinjiang and Ningxia, Jilin, And the most study results is Negative. Currentresearch on Mongolian in sporadic breast cancer has not reported. So the distribution andexpression of study of BRCA1is a need for women in Inner Mongolia in sporadic breastcancer in differences between the Han. Solid growth, proliferation and metastasis ofmalignant tumors cannot be achieved without adequate nutrition, Rich in nutrients mustbe provided by a large number of vessels, Or hypoxia and necrosis occurs. So,The new blood vessels is the infinite source of proliferation, invasion and metastasis of malignanttumor cells.The Breast cancer is no exception. In recent years vascular endothelial growthfactor (VEGF) was discovered the most powerful, most directly promote the proliferationof vascular endothelial cell growth factor; It can promote the Formation of tumorangiogenesis new blood vessels and creating the conditions for growth, invasion andmetastasis of tumor. micro vessel density (MVD) are important indicators of detection oftumor blood vessel activity. Two factors combined Study on invasion and metastasis ofcancer is hotspots for far the most scholars.but, three factors joint research amongBRCA1, there is no report at home and abroad. Therefore, this study byimmunohistochemistry(IHC), the expression of tumor suppressor genes BRCA1, VEGFand MVD were detected in sporadic breast cancer in the Midwest region of InnerMongolia the Mongolian and Han Chinese of Inner Mongolia, combined with clinicaland pathological data to explore three of the Mongolian the difference between theexpression characteristics of sporadic breast cancer in women and breast cancer with theHan and mutual relations, with the metastatic potential of the Mongols in sporadic breastcancer diagnosis, treatment, looking for new ways will have clinical treatment andprognosis of the individual the judgment has important significance.This is the purpose ofthis study and innovation.Methods: In this study, by immunohistochemistry (IHC) method (SP method), to selectthe Midwest region of Inner Mongolia of baotou, Hohhot and Ordos gold centers in theregion from January in2007to October in2011,32cases of Mongolian and52cases ofHan female patients with sporadic breast cancer paraffin-embedded specimens to detectBRCA1and VEGF expression and MVD in their organizations, and22cases ofMongolian women and20cases of Han women benign breast tumor, adjacentnon-cancerous normal tissue as control, and analysis of three the relationship between thecharacteristics and correlation of expression differences in the Mongolian, Han Chinesewomen and its relationship with clinicopathological parameters.Results:32cases detected Mongolian sporadic the Invasive ductal carcinoma of thebreast(BIDC) organizations in11cases of deletion of the BRCA1protein expression in21cases of positive expression,the positive expression rate was65.62%,52casesdetected Han sporadic the BIDC organizations in22cases of deletion of the BRCA1 protein expression,30cases were positive, the positive expression rate was57.69%,showing that the BRCA1protein in the Mongolian breast cancer positive expression rateof65.62%than higher the positive expression rate of57.69%in the Han breast cancer inBRCA1expression, but in two inter-ethnic differences not statistically significant (P>0.05). The positive expression rate of BRCA1in Mongolian and Han Chinese breastcancer tissues than higher in adjacent non-cancerous normal tissues and benign diseasetissues, The positive expression rate of BRCA1in han female patients were57.69%,80%,100%in the three types of organizations, the positive expression of BRCA1inMongolians female patients were65.62%,90%,100%in the three types of organizations,showed an increasing trend, the differences were statistically significant (P<0.05).respectively, in adjacent non-cancerous normal tissues and benign disease tissues positiveexpression rate of BRCA1inter-ethnic differences not statistically significant (P>0.05).Expression of VEGF and MVD positive rate in Mongolian and Han in breast cancertissue was significantly higher than benign breast disease, carcinoma and adjacentnon-cancerous normal breast tissue, the positive expression rate of the Mongolian VEGF81.25%,41.67%,40%and MVD for31.24±7.14,17.97±2.78,10.80±3.38, HanVEGF was80.77%,60%,40%, MVD was30.63±6.78,15.29±2.56,9.83±1.27,both positive expression showed an increasing trend, the difference statisticallysignificant (P<0.05). VEGF and MVD were positive in tissue of the breast Cancer,adjacent non-cancerous and benign disease in the three types of organizations betweenthe two ethnic expression rate was no significant difference (P>0.05). BRCA1expressionof Mongolia and Han with lymph node metastasis was a negative correlation, thedifference was statistically significant (P<0.05). BRCA1expression of Mongolia andHan With patient age, tumor size, ER, PR, Her-2and the side of the other there was nocorrelation. In Mongolian and Han expression of VEGF, was no correlation with patientage, tumor size, ER, PR, Her-2and the side of the other there, With clinical stage,histological grade and lymph node metastasis the Han VEGF expression was a positivecorrelation, the difference was statistically significance (P<0.05). Mongolian VEGFexpression only correlated with lymph node metastasis, were not correlated with variousother. Mongolian and Han ethnic MVD expression with patient age, clinical stage,histological grade, tumor size, lymph node metastasis, ER, PR, Her-2and the side of the other was no correlation with statistical difference (P>0.05). But The characteristics ofthe MVD in the two ethnic expression in the clinical stage, histological grade, tumor size,lymph node metastasis showed an increasing trend by statistical, the clinical stage inPhase I, II, III, during the organization classification class I and II, class III, and no lymphnode metastasis in Han nationality, there were significant differences (P<0.05), while theMongolian Apart from these aspects have a significant difference, in tumors<2cm and>5cm between statistical difference(P<0.05). Protein expression correlation analysisshowed that the Mongolian, Han breast cancer tissue of negative group was significantlyhigher than the positive group, But, the expression of BRCA1and VEGF, MVDexpression both showed a negative correlation, Mongolian, Han Chinese in breast cancertissue VEGF protein expression and MVD was positively related to the differencestatistically significant(P<0.05). Various pathological features were no significant ininter-ethnic Mongolian and Han.,Conclusion: through the research of this topic can be drawn, In the Midwest InnerMongolia, The non-expression rate of BRCA1gene of Mongolia and Han nationalityfemale patients in sporadic breast cancer was high. BRCA1in adjacent non-cancerousnormal tissues and breast benign tumor tissue expression rate is high. The expression rateof VEGF and MVD in breast cancer is high than in adjacent non-cancerous normaltissues and breast benign tumor tissue. Mongolian and Han minority female patientsBRCA1expression and lymph node metastasis were negatively correlated, Mongolianand Han minority female patients VEGF, MVD expression and lymph node metastasiswere Positively correlated. Mongolian and Han nationality female patients the expressionof BRCA1in tissue of breast carcinoma and the expression of VEGF, MVD werenegatively correlated, while the expression of VEGF protein was positively correlatedwith MVD, all expression results in ethnic groups were not statistically different, namelyand national independent.That the absence of BRCA1expression and VEGF, MVD highexpression in sporadic breast cancer suggests tumor cell invasion, early metastasis,malignant degree, poor prognosis. So the high expression of BRCA1could suppressVEGF angiogenesis effects, thus, slow down the rapid invasion and metastasis of tumorcells, improve the survival rate, clinical prognostic judgment has important significance.