| Purpose: using microare oxidation technology (microare oxidation, MAO),through the control micro-arc oxidation the processing time, after polishing andcleaning business pure titanium surface won respectively1-5μ m and-10of the apertureand the corresponding μ m thickness of ceramic oxidation film. Through the osteoblastdifferent diameter and thickness of the ceramic film adhesion effects, discussesmicro-arc oxidation technology in titanium plant body surface modification of value.For clinical research immediate or early planting body weight with more parameters.Method: buy15mm with sides,2mm thickness of the finished product of puretitanium square of72pieces, the surface after water sand paper burnish, removal ofsurface titanium hydrofluoric acid oxidation film, acetone ultrasonic washing anddrying in reserve. Remove48pieces was immersed in electrolyte configured phosphate,through the control processing time won respectively1-5μ m and6-10of the apertureand the corresponding μ m thickness of ceramic membrane of the titanium oxide the therockets.the three groups for the cell experiment:1,MC-3T3osteogenesis cell culture in the material surface after inoculation,scanning electronic microscope specimens, the morphological observation and the cellcount.2, the CCK-8cell count method standard curve cell density estimate, detection cellactivity and appreciation.3, ALP activity determinationResults:1. Micro-arc oxidation film aperture the bigger the cells to the body.2. Cells in large diameter oxidation film on the best value. 3. In large diameter micro-arc oxidation film cells on ALP activity is the strongest.Conclusion:1and after micro-arc oxidation process after the pure titanium biocompatibility ofsignificantly higher than did not handle the pure titanium pieces.2, micro-arc oxidation aperture of the oxide film after the more conducive to cellgrowth... |
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