The Effects Of Ginkgo Biloba Extract (EGb761) On The Neuron Differentiation Of Human Adipose-Derived Stem Cells (hADSCs)

Objective: To evaluate the effects of Ginkgo biloba Extract (EGb761) on theproliferation and the neuron differentiation of Human Adipose-Derived Stem Cells(hADSCs).Methods: Using the CCK-8assay for the detection of different concentration ofEGb761(40mg/L,80mg/L,120mg/L) on the hADSCs proliferation; containingdifferent concentration of EGb761(40mg/L,80mg/L,120mg/L) combined neuralinducing factor (20ng/ml bFGF,20ng/ml EGF,20ng/ml BDNF) on hADSCs toinduce the neuron differentiation. Observation of the morphological changes;Immunofluorescence detection of Nestin, NSE, GFAP to3D,7d,10d,14d expression,counting cells positive rate.Results: EGb761on the proliferation of hADSCs has the time difference,1d hasno significant difference,3d has remarkable promotion,5d has romote flattenedproliferation, cells proliferation rate reduce on7d; containing various concentrationEGb761induced by a combination of factors to promote hADSCs morphological neuraldifferentiation，compared with the positive control group at each time point ofpromoting the expression of Nestin, and the concentration dependence of the trend；promoting NSE expression, no obvious concentration dependence；the expression ofGFAP positive control with no difference.Conclusion EGb761on the hADSCs proliferation has promoting effect,especially on3d; EGb761significantly promoted hADSCs neural differentiation, do notpromote to glial cell differentiation.