The Changes And Effect Of T Lymphocyte Subsets In Peripheral Blood Of Un Treated Patients With Spinal Tuberculosis

Objective:By detecting the changes of T lymphocyte subsets, IFN-y and IL-4in peripheral blood of patients with spinal tuberculosis in the different time, to explore the immunity change rule of patients with spinal tuberculosis, and to provide a certain basis for establishing and adjusting the individualized treatment of spinal tuberculosis.Materials and methods:Both absolute counts and percentages of lymphocyte subsets in peripheral blood of the case group of51patients with treated firstly were detected by single-plate form flowcytometry, and compared with the healthy control group. There are36cases by operated. After operation1to3days and1month, the T cells subgroup detection were compare with the preoperative. In these cases, single nuclear cell plasma were extracted, and the samples were kept at low temperature.IFN-γ, IL-4and protein levels were detected by flowcytometry, and were analyzed in the different time.Results:In this group of patients with spinal tuberculosis, both absolute counts and percentages of lymphocyte subsets of CD3+and CD4+are lower than healthy control group. The differences were statistically significant (P<0.05) between two groups. But there were no statistical difference (P>0.05)in the absolute counts and percentages of lymphocyte subsetsofCD8+, CD19+and NK cells between two groups. Compared with before and after surgery the group, CD4+T cell levels of T cell subgroup further reduced (P<0.05), and the rest (including CD3+, CD8+, CD19+and NK cell) had no statistical differences (P>0.05). there were statistical difference (P<0.05) between postoperative1month and before treatment in the absolute counts and percentages of lymphocyte subsetsCD3+and CD4+T cells,but the results were still lower than the normal. Cytokines showed that IFN-y protein level of patients with spinal tuberculosis befor etreatment was lower than the healthy control group. The difference has significant statistical significance (P<0.01), but there was no statistical significance (P>0.05) in IL-4protein level between two groups. The protein levels of IFN-γ and IL-4were no obvious change after operation. The level of IFN-γ had been improved after surgeryl month in patient group, but it still was lower than normal (P<0.05).Conclusion:T lymphocyte immune function in patients with spinal tuberculosis decreased. The absolute count and percentage of CD4+lymphocytes decreased more obviously. At the same time, cytokines expression of IFN-y which was mainly the secretion of CD4+T lymphocyte had also been obvious inhibition. Operation stress can further reduce the body’s immunity. The levels of T cells and cytokines IFN-γ and IL-4changes with different treatment period,. It can better reflect immune state, and provide important reference for the establishment of individualized treatment plan.