Prognostic Research Of Transgelin、MMP-7and HMGB1Protein Expression In Rectal Cancer

Background and Objective：The prognosis of Rectal cancer is related to many factors，age，Duke’s stage，histology Lymph node metastasis, depth of invasion etc. However intreatment we found that even patients have the same prognostic factors and treatment，their prognosis also different. This point out that maybe some unknown factors effectthe prognosis. therefore，the aim of this study was to assess the relationshipbetween the expression of the Transgelin, MMP-7and HMGB1protein in Rectal cancerwith theirs clinical pathological characteristics and prognostic significance and theirprotential relevance. Providing theoretical foundation for the treatment and prognosis ofRectal cancer.Methods：From2006to July2009，66cases with Rectal cancer.were recuited in thisstudy. Collected their paraffin embedded specimens from pathology department. Therewere45male and21female with the median age of55years(range30-80years).Transgelin, MMP-7and HMGB1protein expression were detected in Rectal cancertissues by Elivison immunohistochemistry. Statistical anaysis was perormed using SPSSstatistical software package(version13.0).Survival curves were calculated according toKaplan-Meier method and compared with the use of Log-rank test.Multivariate analysiswas based on Cox regression model with P values lower than0.05considered asstatistically significant.Results：Transgelin, MMP-7, HMGB1positive expression rate in rectal cancer were51.6%,40%,47%of Transgelin positive expression of gender, age, pathologicaldifferentiation type, Duke’s stage, depth of invasion, lymph node metastasisno correlation, its negative group overall survival was shorter. The positive expression ofMMP-7, respectively, with Duke’s stage, pathological type, depth of invasion, gender,age, and so no correlation with lymph node metastasis, the pathological differentiationrelevance. HMGB1expression and pathological differentiation, depth of invasion ofcorrelation with Duke’s stage, lymph node metastasis, gender, age no correlation.MMP-7, HMGB1correlation in invasion and metastasis in rectal cancer, has a strongpositive correlation. Transgelin, MMP-7, HMGB1no correlation. The whole group ofdeath in22cases, to survive44cases, the median survival time of61months (16-79months) of the survival time,3years. The5-year survival rates were81%、69.7%.Univariate analysis showed: age, Duke’s stage, depth of invasion, pathologicaldifferentiation type of lymph node metastasis, MMP-7, HMGB1, Transgelin survival ofrectal cancer patients influential (P <0.05), Duke’s stage, depth of invasion of rectalcancer independent prognostic factor in patients (P <0.05).Conclusions：1．MMP-7in different pathological types of cancer tissue differentiation and expressionof HMGB1different, the difference was statistically significant expression of HMGB1significantly improved with the change of the degree of differentiation of MMP-7,HMGB1, MMP-7correlated with rectal cancer survival rates, a reference to determinethe prognosis of patients.2．MMP-7in cancer tissues was positively correlated with HMGB1.3．Age, Duke’s stage, depth of invasion, histological type of differentiation, lymph nodemetastasis, MMP-7, HMGB1, can of Transgelin be as a predictor of cancer patientsprognosis reference index.