| Background:Oral squamous cell carcinima(OSCC) which accounts for80%of the oralmalignant tumors is the most common malignant tumor in oral and maxillofacial,itsincidence has a rising trend in the world. Currently,the main treatment method issurgery excision, and the adjunctive treatment is radiotherapy and chemotherapy. Butthe ability of early invasion and metastasis of oral squamous cell carcinoma ispowerful,so the patient’s prognosis is very poor. If certain biochemical markers can befound before its formation, then active and effective treat is gived,the cancerousprocess is blocked at the precancerous stage, finally,the cure rate be greatly improved.Matrix metalloproteinases (MMP-7) and basic fibroblast growth factor (bFGF) are twoimportant tumor markers for oral squamous cell carcinoma and they play importantrole in tumor development. With the development of corresponding detectiontechnology, according to oral squamous cell carcinoma markers existing in patient’sblood, urine or other body fluids, we can not only assist in the early diagnosis of oralsquamous cell carcinoma, but also obtain effective information for its therapeuticeffect, prognostic judgment, postoperative adjuvant treatment programs.Purpose:This study was designed to investigate the mRNAexpression of MMP-7and bFGFin the specimen of oral squamous cell carcinoma and normal oral tissue frommolecular level,and we also analysed the correlation of MMP-7and bFGF withpatients’ the clinical and pathological features,such as age, sex, tumor size, lymphnode metastasis and pathological stage, in order to explore their role in thedevelopment of oral squamous cell carcinoma and their mutual relationship,So as to provide effective clinical work auxiliaryMethods:In the study,36cases of oral squamous cell carcinoma were selected as theexperimental group, and14cases of normal mucosas tissue were selected as the controlgroup. The specimens of the experimental group were selected from February2011toApril2012, which were surgically excised in our department and were confirmed bythe department of pathology in our hospital. RT-PCR was used to the expression ofMMP-7and bFGF mRNA in the experimental group and the control group, then weanalyze the results combing with clinical and pathological data. All samples mustfollow the standards: complete clinical and pathological data,the first cases, definitediagnosis, no other head and neck cancer history, no radiotherapy and chemotherapyhistory.Result:1. The positive expression rate of MMP-7mRNA in OSCC was77.8%（28/36）, higherthan that in NOT which was14.3%（2/14）, and the difference of the rate is statisticallysignificant(P＜0.01). The positive expression rate of bFGF mRNA in OSCC was83.3%（30/36）, higher than that in NOT which was21.4%（3/14）, and the differenceof the rate is statistically significant(P＜0.01).2. The expression of MMP-7mRNA in OSCC had positive correlation with lymphnode metastasis and clinical stage(P＜0.05), but no correlation with patients’ age andgender(P>0.05). The expression of bFGF mRNA in OSCC also had positivecorrelation with lymph node metastasis and clinical stage(P＜0.05), but did notcorrelate with patients’ age, gender (P>0.05).3. Significantly correlation between MMP-7and bFGF mRNA expression wasobserved in OSCC(r=0.621，P<0.01).Conclusion:The abnormal expressions of MMP-7and bFGF are closely related to the biological behavior of OSCC, and both of them are participated in the invasion andmetastsis of OSCC, they play some kind of synergy during this process.So they maybecome markers in predicting the invasion and metastasis of OSCC and provideevaluation criterions for the patients’ prognosis. |
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