The Expression Of PECAM-1 Gene In Oral Verrucous Carcinoma

Objective To study the expression the platelets - endothelial cell adhesion molecule(PECAM-1)in oral verrucous carcinoma(OVC)and oral squamous cell carcinoma(OSCC),so as to investigate the role of this gene in the development of OVC and OSCC,and to validate the results of gene chip.Methods 9 cases of exogenic OVC,6 cases of cystoid and infiltrative OVC,15 cases of well/moderately- differentiation OSCC (w/md-OSCC)and 15 cases of poor-differentiation OSCC(pd-OSCC) were selected;two specimens,which were carcinoma tissues and the surround normal tissues,were taken from the same patients.Test the mRNA expression of PECAM-1 gene by reverse transcription polymerase chain reaction(RT-PCR)and check the PECAM-1 protein by immunohistochemistry(IHC)in the above specimens.Results1.PECAM-1 of the IHC testa.The microvessel density(MVD)of PECAM-1 in 2 types OVC and 2 types OSCC was higher than normal tissues(P＜0.05).b.There was difference of PECAM-1's MVD between w/md-OSCC and pd-OSCC(P＜0.05). c.The MVD of PECAM-1 was significantly higher in this order:Exogenic verrucous carcinoma,Well differentiated squamous cell carcinoma,Cystoid/infiltrative verrucous carcinoma,Poorly differentiated squamous cell carcinoma.(P＜0.05).2.PECAM-1 of the RT-PCR testa.The mRNA expression of PECAM-1 gene was significantly higher in both exogenic OVC and cystoid and infiltrative OVC than in their matched normal tissues(P＜0.05);The mRNA expression of PECAM-1 gene was also significantly higher in both w/md-OSCC and pd-OSCC than in their matched normal tissues(P＜0.05).b.There was difference in the mRNA expression of PECAM-1 gene between w/md-OSCC and pd-OSCC(P＜0.05).c.The mRNA of PECAM-1 was significantly higher in this order:Exogenic verrucous carcinoma,Well differentiated squamous cell carcinoma,Cystoid/infiltrative verrucous carcinoma,Poorly differentiated squamous cell carcinoma.(P＜0.05).Conclusion1.PECAM-1 may play an key role in the invade and metastasis of OVC and OSCC,Of which promote the formation of tumor blood vessels is the main mechanism for the pathogenesis. 2.The difference of PECAM-1 in OVC and OSCC may be the gene foundation of invade and metastasis that the potential of OVC is lower than OSCC.3.And it also showed part of cystoid/infiltrative OVC can have a higher potential of invade and metastasis than OSCC.