Study On Steroidal Saponins From Ophiopogon Japonicus

Objective:Ophiopogon japonicus Ker-Gawl.(Liliaceae), an evergreen perennial, has been widely cultivated in mainland China, especially in Sichuan and Zhejiang provinces. The tubers of O. japonicas have been widely used as a traditional Chinese medicine for treating cardiovascular diseases and chronic inflammatory diseases. A large number of literature reports indicate that steroid saponins have many actives in it. Such as anti-arrhythmic, anti-carcinogenic, anti-inflammatory, anti-shock and anti-aging, so it has high research valie and development potential. As part of a continuing study of the steroidal saponins from O. japonicas, expect to get more new compound to enrich steroidal saponins, and look for compounds with potential for development.Methods:On the basis of extensive spectroscopic analysis, including1D (1H and13C NMR) and2D NMR (COSY, HSQC and HMBC) data, MS data and the results of acid hydrolysis, compounds1to15were determined as new steroid saponins.Results:The structures of24compounds were as follows:Ophiopogonin P (1), Ophiopogonin Q (2), Ophiofurospiside C (3), Ophiofurospiside D (4), Ophiofurospiside E (5), Ophiofurospiside F (6), Ophiofurospiside G (7), Ophiofurospiside H (8), Ophiofurospiside I (9), Ophiofurospiside J (10), Ophiofurospiside K (11), Ophiofurospiside L (12), Ophiofurospiside M (13), Ophiofurospiside N (14), Ophiopogonin R (15),(25R)-26-O-β-D-glucopyranosyl-3β,22α,26-trihydroxy-furost-5-ene-3-O-α-L-rhamno pyranosyl-(1â†'2)-[β-D-xylopyranosyl-(1â†'4)]-β-D-glucopyranoside(16),(25R)-26-O-β-D-glucopyranosyl-3β,22α,26-trihydroxy-furost-5-ene-3-O-α-L-rhamnopyranosyl-(1â†'2-β-D-glucopyranoside (17), ophiofurospiside A (18), Ophiopogonins H (19),(25R-26-O-[β-D-glucopyranosyl-(1â†'6)-β-D-glucopyranosyl]-3β,17α;22a,26-tetrahydroxy-furost-5-ene-3-O-α-L-rhamnopyranosyl-(1â†'2)-β-D-glucopyranoside (20),25R-spirost-5-ene-3β,17α-dihydroxy-3-O-α-L-rhamnopyranosyl-(1â†'2)-[β-D-xylopyranosyl-(1â†'4)]-β-D-glucopyranoside (21),Tb (22),22S-cholest-5-ene-1β,3β,16β,22-terol-1-O-α-L-rhamnopyranosyl-16-β-D-glucopyranoside (23),22S-cholest-1β,3β,16β,22-terol-1-O-α-L-rhamnopyranosyl-16-β-D-glucopyranoside (24).Conclusion:This paper reports the isolation and structural elucidation of29steroidal saponins, include10furostanol saponins with the disaccharide chain linked at C-26hydroxy group of the aglycone have been rarely reported from natural sources. compounds1to15were determined as new steroid saponins.