Study On The Chemical Compositions Of Ferula. Sinkiangensis

Objective: Ferula sinkiangensis K. M. Shen is a popular drug in Uygur. It was recordin Chinese Pharmacopoeia （2010）. To study the TLC character of Ferula sinkiangensisaccording to the TLC method（Chinese Pharmacopoeia （2010） appendix â…¥B）, by usingdifferent extraction methods, choosing right developing solvent, coloring way and viewingway. To establish TLC identifying method of chemical component of Ferula sinkiangensis.To determine the content of ferulic acid in10batches of Ferula sinkiangensis. To establishHPLC fingerprint of Ferula sinkiangensis K. M. Shen. To determine the total antioxidantactivity of Ferula sinkiangensis by UV-DPPH. To study the absorption of Ferulasinkiangensis. Methods:1. Ferula sinkiangensis were respectively extracted by diluteHCl or carbinol; unfolded by cyclohexane-dichlormethane-glacial acetic acid （8:8:1）,methylbenzene-ethyl acetate （10:3） or normal hexane-petroleum ether （19:1）; colored by1%FeCl3ethanol-1%K3Fe（CN）6or iodine vapor; inspected at366nm or visible light.2.Agilent Prep-C₁₈Scalar chromatographic column was choosed, acetonitrile-0.1%phosphoric acid （14:86） was moving phase, flow rate was1.0mL/min, determinewavelength was323nm, sample amount was10μL.3. The gradient elution program wasestablished, The HPLC result of10batches of Ferula sinkiangensis were analysed byChinese Medicine Chromatographic Fingerprint Similarity Evaluation System （2004A）and ChemPattern1.51.4. The absorbancy of reaction between Ferula and DPPH wasdetermined by UV-VIS, EC50was calculated. Antioxidant activity of Ferula was viewedby DPPH after unfolded by two development system, allowing the extracting method ofChinese Pharmacopoeia （2010）.5. After the gavage of freeze-dried powder to the rats,blood was collected from hepatic portal vein, centrifuged, determined afterdeproteinization. Result:1. Of the extractions of HCl,9spots were caught beforecoloration, and4spots were caught after coloration. Of the extracts of acetic ether,equilibrated by methylbenzene-ethyl acetate （10:3）,13spots were caught beforecoloration, and10spots were caught after coloration; equilibrated by normal hexane-petroleum ether （19:1）,2spots were caught after coloration.2. There is derefence of content of Ferulic acid among10batches of Ferula sinkiangensis.3. The condition ofHPLC fingerprints of Ferula sinkiangensis was established.4. The EC50of methyl alcoholextraction of Ferula sinkiangensis to DPPH is1.67mg·mL^-1.5. The absorbing feature ofFerula sinkiangensis was known preliminary. Conclusion: The TLC identifying featuresprovided methods for quality control of Ferula sinkiangensis. The HPLC fingerprints ofFerula sinkiangensis provided identifying standards for Ferula sinkiangensis, is the basicof establishing standards of A-Wei capsules and other relevant prescriptions. Theantioxidant material of Ferula sinkiangensis can be determined by TLC-DPPH method.