Experimental Study On Spinal Cord Injury Of Different Programs Spinal IGRT By Evoked Potential Detection

Objective: Optimize programs of spinal (image guided radiation therapy)IGRT byEvoked Potential detection in spinal cord injury make further efforts of the value ofevoked potential in the spinal cord injury diagnosis is confirmed by the apoptosis index.Methods: Choose male adult36beagles, a random number table method randomlydivided into3groups, each group of12beagles, repeated experiment. L12adoptorthogonal experiment design in each group (3×2~3),, and in the radiotherapy plan(radiation beam,5,7,9wild3level; divided doses16Gy,20Gy2level; the dose rate4gy/min,6gy/min2level; rays Angle, ranging from2level) to examine factors and levels.Simulated7~12thoracic vertebrae tumor, within5w give it7-12thoracic vertebral bodyradiation. Plasmids are evoked potential: leather (cortical somatosensory evoked potential,CSEP) and motor evoked potential (motorevoked potential, MEP) as evaluation indexdetection of spinal cord injury, spinal IGRT scheme of optimization is excellent And takenthe dog the same segmental spinal cord (thoracic7-12)in3months after radiotherapyliving materials. Using TUNEL staining to observe spinal cord nerve cells apoptosis andcalculated. apoptosis index. Results:(1)The TUNEL apoptosis index test results andCSEP and MEP as an index screening is consistent with same optimal schedule;(2)Thereliability of evoked potential in the spinal cord injury diagnosis is confirmed by theevoked potentials terminal measurements and the apoptosis index;(3)The maininfluencing factors of the radiation treatment plan is divided doses, followed by the doserate, radiation field and the radiation Angle.Conclusion:(1) The evoked potential ofMEP and CSEP can be used as the of observation indicators; radioactive spinal cordinjury.(2) the result of optimal schedule: the divided doses is16Gy; The dose rate is4Gy/min; the radiation field is9field; the radiation angle is equal angle.