| [Objective]Exogenous S.s was inoculated to animal models of beagles with peri-implantitics. The changes of the clinical indicators of peri-implantitics were observed and the changes of the subgingival micro flora were detected through the real-time quantitative PCR. That whether inoculation of exogenous S.s would change the composition of peri-implant subgingival microflora was explored to find a new way to treat peri-implantitics.[Methods]7dental implants in the4beagles on which peri-implantitics models had been established were randomly divided into group A, B. C and D. Group A:The controlled group without any treatment; Group B:Resin curette for peri-implant and periodontal curette for other teeth to conduct supragingival scaling and subgingival scaling; Group C:The same treatment as in group B; and then on that same day after scaling, the inoculation of exogenous S.s for a single time was conducted; Group D: The same treatment as in group B; and then on the same day after scaling and at the1st,2nd and4th week, the inoculation of exogenous S.s for single time were conducted, respectively. Peri-implant clinical indicators such as PD, BOP, mGI, mobility and bone height loss were measured at the baseline and at the1st,2nd,4th,8th and12th weeks in each group, respectively. Proportions of S.s, F.n, P.g and P.i at the baseline and at the1st,2nd,4th,8th and12th weeks in each group were detected through the real-time quantitative PCR method, respectively.[Results]1. Influence of inoculation of exogenous S.s on clinical indicators of peri-implantitics in beagles. Differences of baseline PD among the groups had no statistical significance (P>0.05), and the PD difference at each observation points in group A also had no statistical significance (P>0.05). While compared to the baseline, PD at the1st,2nd,4th,8th and12th week of group B, C and D were decreased and the differences were statistically significant (P<0.05), however, PD differences among group B, C and D had no statistical significance (P>0.05).Baseline BOP in each group was100%. At the1st,2nd,4th,8th and12th week, BOP differences between group A and B had no statistical significance (P>0.05). At the2nd,4th,8th and12th week, BOP of group C and D were decreased when compared with baseline and the differences had statistical significance (P<0.05). At the4th and12th week, BOP in group D was lower than that of group B, and the differences had statistical significance (P<0.05).Through the rank sum test, mGI index of group B, C and D at the4th,8th and12th week were decreased when compared with baseline (P<0.05), there was no statistically significant differences among the groups (P>0.05).Differences of mobility of dental implant and bone height loss of periapical film in group A, B. C and D at each observation points were not found obvious difference.2. Influence of inoculation of exogenous S.s on subgingival microflora of peri-implantitics in beaglesDifferences of baseline proportions of S.s among groups had no statistical significance (P>0.05). Proportions differences of S.s in group A had no statistical significance at all the observation points (P>0.05). At the1st,2nd,4th and8th week after baseline, S.s proportions in group B, C and D were higher than the baseline, and the differences were statistically significant (P<0.05). At the1st,2nd,4th,8th and12th week, S.s proportions in group B were lower than those in group C and D, and the differences were statistically significant (P<0.05). At the4th,8th and12week, the S.s proportions in group D was higher than those in group C, and the differences were statistically significant (P<0.05). At the12th week, it was found that there was no statistically significant difference between the S.s proportions in group A and B (P>0.05).There were no statistical significance of the difference among the baseline proportions of P.g. P.i and F.n (P>0.05). Proportions of P.g, P.i and F.n in group A at all the observation points were not statistical different (P>0.05). At the1st,2nd,4th and8th week in group B, proportions of P.g, P.i and F.n were reduced when compared with baseline, and there was statistical significance on the differences (P<0.05). At the12th week, there was no statistical significance for the difference of proportions of P.g, P.i and F.n, when compared with group A (P>0.05). At the1st,2nd,4th,8th and12th week, proportions of P.g, P.i and F.n in group C and D were both lower than the baseline and the differences were of statistical significance (P<0.05). At the12th week in group D, proportions of P.g, P.i and F.n were lower than those in group C, and the differences had statistical significance (P<0.05).[Conclusion]Peri-implant initial therapy could improve the clinical indicators PD and mGI of peri-implantitics in beagles effectively, and auxiliary application of exogenous S.s could improve BOP; Under the circumstance without loading, the mobility of dental implant was not changed; During the whole experiment process, bone heights of dental implants in each groups had not changed; Exogenous S.s could inhibit periodontal putative pathogen such as peri-implant F.n, P.g, Pi, etc.; At the12th week after initial therapy, peri-implant subgingival microflora almost recovered to the level before treatment; Repeated inoculation of exogenous S.s had a longer inhibition duration to peri-implant F.n, P.g and Pi than the single inoculation; but action duration of single inoculation was unclear. Repeated inoculation of exogenous S.s could be served as an adjuvant treatment after initial therapy to peri-implantitics in beagles. |
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