| Objective:To collect oral saliva of patients with CHF,purify and culture the Streptococcus sanguis strains in saliva,and determine the total number of Streptococcus sanguis strains,so as to preliminarily explore the correlation between patients with chronic heart failure and non heart failure control group,as well as between different TCM Syndromes of chronic heart failure.Methods:From March 2019 to May 2020,all the cases came from the patients with CHF who were treated in Nanjing Chinese Medicine Hospital.64 patients with CHF who met the inclusion and exclusion criteria were selected as the observation group.They were divided into the syndrome differentiation group of Qi Yin deficiency and blood stasis syndrome group(31 cases)and Yang Qi deficiency and blood stasis syndrome group(33 cases)and non CHF patients were the control group(30 cases).Collecting the general information of patients' name,gender,age,NT-proBNP in serum and the risk factors related to CHF,and taking the saliva of patients for the cultivation of Streptococcus sanguis.To observe the detection results of the number of Streptococcus sanguis in saliva,and study the difference of Streptococcus content between different TCM Syndromes of CHF and between CHF patients and control group.Results:64 cases of CHF were finally included in this study,and they were divided into two groups according to TCM syndrome differentiation:(1)31 cases of Qi Yin deficiency and blood stasis syndrome group,including 20 males and 11 females,with an average age of(69.84±8.97)years old;(2)33 cases of Yang Qi deficiency and blood stasis syndrome group,including 20 males and 13 females,with an average age of(77.12±7.42)years old.In addition,30 cases were included in the non CHF group,including 15 males and 15 females,with an average age of(73.33+4.73)years old.The number of Streptococcus sanguis in saliva of the patients with CHF was higher than that of the control group(353.52±13.43*108cfu/L),and the number of Streptococcus sanguis in saliva of the patients with Yang Qi deficiency and blood stasis syndrome group was higher than that of the patients with Qi Yin deficiency and blood stasis syndrome group,(345.81±12.29*108cfu/L),which was higher than that of the control group(304.30±38.57*108cfu/L)(P<0.05).The number of oral Streptococcus sanguis in patients with coronary heart disease(352.77±11.46*108cfu/L)was higher than that in patients without coronary heart disease(345.12±14.96*108cfu/L),the difference was statistically significant(P<0.05).There was a positive correlation between the number of oral Streptococcus sanguis and NT proBNP in patients with chronic heart failure.The number of Streptococcus sanguis in oral(353.613±9.52*108cfu/L)of patients with CRP>10mg/L was higher than that of patients with CRP?10mg/L(346.18 ± 15.47*108cfu/L),which was statistically significant(P<0.05),suggesting that there might be a certain correlation between Streptococcus sanguis and CRP.Conclusion:(1)The number of Streptococcus sanguis in saliva of 64 patients with CHF was higher than that of 30 patients without CHF control group,and the number of Streptococcus sanguis in saliva of 34 patients with Yang Qi deficiency and blood stasis syndrome group was higher than that of 31 patients with Qi Yin deficiency and blood stasis syndrome group.(2)Among the 64 heart failure patients included in the study,hypertension and coronary heart disease accounted for a relatively high proportion.There was a correlation between coronary heart disease and the number of Streptococcus sanguis in saliva.The number of Streptococcus sanguis in saliva of 39 patients with coronary heart failure was higher than that of 25 patients without coronary heart failure,the difference was statistically significant(P<0.05).The results may be related to the risk factors of atherosclerotic plaque caused by Streptococcus sanguis.(3)There was a positive correlation between the number of Streptococcus sanguis in saliva and NT-proBNP in patients with CHF,which initially reflected that there was a certain correlation between the number of Streptococcus sanguis in saliva and the severity of heart failure in patients with chronic heart failure.Patients with severe heart failure may be more likely to have oral microbial disorders.(4)In patients with CHF,the number of Streptococcus in oral blood of patients with CRP>10mg/L is higher than that of patients with CRP?10mg/L.The preliminary analysis of the results is related to the theory of inflammatory mediators described in the literature,and the risk of abnormal CRP value in patients with heart failure is increased. |
PDF Full Text Request