The Study On Fingerprint-efficacy Relationship And The Main Anticoagulation Components Of Panaxnotoginseng From Different Resources

The study was aimed to develop the fingerprint-efficacy of P. notoginseng,finding the relationship between fingerprint and anticoagulation effect, establishing anintegrated evaluation system, and determining the main active components in P.notoginseng fingerprints. Then, the evaluation of the anticoagulation effect of PTS,PDS and PNS from P. notoginseng on dose-effect relationship was conducted. Inaddition, we also compared the anticoagulation effect of flowers and leaves with rootsof P. notoginseng. The combination model can be applied to investigate activecomponents for the quality control of P. notoginseng and other TCMs.1. The fingerprint and anticoagulation effect of saponins extracts of P.notoginseng samples. The results showed that these P. notoginseng samples could begrouped into three clusters by hierarchical cluster analysis (HCA) according to theirchemical fingerprints. The samples in Cluster I and Cluster III were all “three years,spring,” whereas Cluster II were not “three years, spring”. This development suggeststhat type and growing year had significant influence on chemical fingerprints andanticoagulant activity. The effects of P. notoginseng samples on anticoagulation wereevaluated by PT assay. The PTs of the samples collected in Cluster II were muchshorter than that of the samples in the other two clusters. This result indicated that“three years, spring” P. notoginseng should be the main medicinal species.2. The establishment of the fingerprint–efficacy relationship of P. notoginseng.The model equation was founded by multiple linear regression analysis (MLRA),which showed that notoginsenoside R1, ginsenoside Rb1, ginsenoside Rd, and anunknown compound might be the major effective components for the quality controlof this traditional Chinese medicine (TCM), presuming that PDS (Rb1, Rd, and theunknown compound) possesses a stronger anticoagulant activity than PTS (R1only).This conjecture was confirmed by the dose–effect relationship evaluation of PDS andPTS. In addition, according to the equation, the peak11strongly affect theanticoagulation of samples with low content while the peak3showed a little impact on anticoagulation with high content, pointing out the main bioactivity components isnot always high content ones.3. The anticoagulation activity of different part of P. notoginseng. The PT assaywas introduced to evaluate the anticoagulant activity of the70%methanol extracts ofroot, leaf and flower of P. notoginseng as well as the various saponins separated fromthe root and leaf by macroporous resin. The results showed that the PT values ofextracts of leaf and flower were significantly above that of root extract. According tothe test of dose-effect relationship,20(S)-protopanaxadiol saponins (PDS) separatedfrom root (50%ethanol extract of root) and leaf (50%ethanol extract of leave)showed a much higher PT value than other samples, especially when theconcentration was under25mg/mL. These results indicate that leaf and flower possessstronger anticoagulant activity among the different part of P. notoginseng. And thePDS from leaf, showed the strongest anticoagulant activity, may be the potentialanticlotting drug.