The Protective Mechanisms Of Erigeron Breviscapus And Panax Notoginseng Extracts On Cerebral Ischemia Reperfusion Injury

Brain stroke, is also named as palsy, and very common cerebrovascular disease, its disability rate and mortality rate are very high. The stroke is divided into two types: hemorrhage and ischemia, among them ischemia is accounted for85%. Oxidative stress and calcium ion overload are involved in its molecular mechansim. Current treatment methods are the dilating of blood vessels, scavenging free radicals, activating blood circulation to dissipate blood stasis.In China’s traditional Chinese medicine, Erigeron breviscapus and Panax notoginseng extracts have been used to treat stroke. They play roles in scavenging free radicals, inhibiting platelet aggregation and ameliorating the brain blood flow and suppressing the neuron death. However,their molecular targets are still unknown.Thioredoxin-1(Trx-1) has a highly conserved sequence and its molecular weight is12kDa. Its active center is,-Cys-Gly-Pro-Cys-. It has a variety of biological functions, for example:regulating redox, regulating transcription factor activity, promoting cell growth and inhibiting cell apoptosis.Heat shock protein70(HSP70) is one of the heat shock protein family, ubiquitiously expressed in various cells and tissues. HSP70is induced under various conditions. HSP70decreases the aggregation of protein and helps protein to fold and turn correctly and inhibits cell apoptosis and regulates the inflammtion.The aim of present study is to clarify the molecualr mechansims on Erigeron breviscapus and Panax notoginseng extracts resisting ischemia by using mice cerebral ischemia model of middle cerebral artery embolization, through peritoneal injection consecutive two days of Erigeron breviscapus and Panax notoginseng extracts, using2,3,5-triphenyltetrazolium chloride(TTC) stain to observe infarction area, by neurobehavioral assessment to check the neurologic deficit, by Western blot to measure protein expression changes. We found that infarct size was significantly decreased in group of administration by Erigeron breviscapus and Panax notoginseng extracts compared with the MCAO group, and behavior deficts were significantly improved. In the hippocampus, MDA was decreased in the hippocampus by adminstration compared with MCAO. The expressions of Trx-1, CDK5and pro-caspase-3were restored by treatment of Erigeron breviscapus and Panax notoginseng extracts compared to MCAO. As same time, decreases of Akt and ERK by ischemia were inhibited by treatment of Erigeron breviscapus and Panax notoginseng extracts.We study showed the roles of Erigeron breviscapus and Panax notoginseng extracts in protecting brain from cerebral ischemia reperfusion injury. These roles are related with Erigeron breviscapus and Panax notoginseng extracts inhibiting oxidative damage, increasing the expressions of Trx-1, CDk5, HSP70. The treatments of Erigeron breviscapus and Panax notoginseng extracts also play roles in regulating pathway on Akt and ERK regulated by MCAO. Our study further clarified molecualr mechansims on Erigeron breviscapus and Panax notoginseng extracts resisting ischemia, provided new treatment targets for cerebreal ischemia.