Association Research Between Environmental Risk Factors，polymorphism Of P21and TNF-α And Pathogenesis To Esophageal Cancer In Kazakh In Xin Jiang

Objective: To investigate the association of polymorphisms of P21and TNF-α andenvironmental Factor in pathogenesis to esophageal cancer family in Kazak in XinjiangProvince. Methods: SNPs in the3’ UTR of P21and TNF-α308were genotyped by thepolymerase chain reaction restriction fragment length polymorphism(PCR-RFLP) analysisin43Kazakh esophageal family and40non-esophageal family，environmental factorswere inquired by questionnaire. Results: The genotype frequencies of TT and TC and CCof3’ UTR of P21in the in Kazakh esophageal family differed from those in the controlfamily(P=0.033，OR=2.502)，The genotype frequencies of TT and TC of P21had2.502times significantly higher in the Xinjiang Kazakh esophageal family，those who comparedwith the Control family。Different genotypes frequencies of P21in esophageal cancerpatients and non-esophageal cancer patients were significantly different，the TT and TCgenotype significantly increased the risk of developing esophageal cancer compared withCC genotype（P=0.004，OR=11.243）. The genotype frequencies of GG and GA and AAof TNF-α308in the in Kazakh esophageal family differed from those in the controlfamily(P=0.004，OR=8.786)，The genotype frequencies of AA and GA of TNF-α308had8.786times significantly higher in the Xinjiang Kazakh esophageal family，those whocompared with the control family。Different genotypes frequencies of TNF-α308inesophageal cancer patients and non-esophageal cancer patients were significantlydifferent，the AA and GA genotype significantly increased the risk of developingesophageal cancer compared with CC genotype（P=0.031，OR=2.821）.When the twogenotype polymorphisms were combined to analyzed， individuals with theTNF-α308GA+AA and P21TC+CC genotypes significantly increased the risk ofdeveloping esophageal cancer，compared with those harboring the TNF-α308GG andP21TT genotypes(P=0.029，OR=10.81)；the two genotype polymorphisms in esophageal cancer patients and non-esophageal cancer patients were no significantly different，P>0.05. The result of analysis of simple factors showed that were not found the variousenvironmental factors and genotypes frequencies of P213’UTR and TNF-α308hadcorrelation (P>0.05). Overeating is correlated with Xinjiang Kazakh esophagealcarcinoma familial aggregation（P=0.021），Drinking, spicy food, less intaking of freshvegetables and fruits are correlated with Xinjiang Kazakh esophageal carcinoma’soccurrences (P=0.022, P=0.009, P=0.044)。Conclusion：The polymorphism of P213’UTR and TNF-α308is likely to be assocated with the high incidence of XinjiangKazakh esophageal family. The polymorphism of the two Gene is likely to play a combineeffect with the high incidence of Xinjiang Kazakh esophageal family. The eating habit ofXinjiang Kazak esophageal family (overeating) is likely to be assocated with the highAggregation of Xinjiang Kazakh esophageal family. Drinking, spicy food, less intaking offresh vegetables and fruits is may be an important risk factor in the EC of XinjiangKazakh.