Study On CYP4502E1 Gene Polymorphism And Related Environmental Risk Factors With Kazakh's Esophageal Cancer In Xinjiang

Objective:1. To the association between CYP2E1 gene polymorphrisms and esophageal cancer(EC) in Xinjiang Kazakh;2. To study CYP4502E1 gene polymorphrism and with risk to develop of Esophageal Cancer(EC) in Kazakh.3. To explore the gene-environment interaction between the CYP2E1 gene polymorphrism and its related metabolic factors with ES in Kazakh.Method:1. Using 1:2 matched case-control study, we have identified 120 esophageal cancer patients with endoscopy and pathology diagnosis from hospital in north of Xinjiang during the periods from March 2005 to June 2007.A total of 240 controls without history of malignant tumor or digestive system diseases were selected during the same period as cases and matched to each case by gender, age, nationality and inhabited areas All the studies conducted on the blood samples and the life behavioral factors epidemiological investigation;2. CYP2E1 genotypes were identified by PCR amplification followed by digestion with RsaI;3. All the calculations and statistics were performed with the computer program, SPSS Version 13.0. Statistical analysises adoptχ2 test, conditional logistic regression to calculate the values ofχ2, odds ratios and 95% confidence intervals for possible risk/protective factors. Check the accordance with Hardy-Weinberg equilibrium in the group.Result:1. The frequencies of C1/C1,C1/C2 and C2/C2 were 53.3%,37.5%and 9.2% in Kazakh's controls and 78.3%,19.2%,2.5% in Kazakh's individuals with EC, The genotype frequencies of EC group is different from controls group (χ2=21.794,df=2,P=0.000). The individuals with C1/C1genotype had a 3.07-fold (95%CI: 1.87～5.03) increased risk of developing EC, The allele C1 of CYP2E1 had a 2.818-fold (95% CI : 1.821～4.360) increased risk of developing EC compared with those who had allele C2.2. Analysis the risk factors related CYP2E1, showed: CYP2E1C1/C1 genotypes,not sanitation of drinking water, excessive smoking,drinking wine ,family history of EC ten factors were risk factors of EC, the use of refrigerators is the protect factor of EC.The use of refrigerators was the protect factor of EC.3. Multivariate conditional Logistic regression analysis showed: not sanitation of drinking water, excessive smoking,drinking wine, eating old grain and pickles, using the refrigerator and CYP2E1C1/C1were important risk factors of EC.4. The interaction shows:Between the CYP2E1C1/C1 genotype and not sanitation of drinking water, excessive smoking,drinking wine, eating old grain and pickles, using the refrigerator have the correlation.Conclusion:1. CYP2E1C1/C1 genotype and diet habits were risk factors of EC in Kazakh of Xinjiang.2.Interaction between gene and entironment showed that:not sanitation of drinking water, excessive smoking,drinking wine, eating old grain and pickles and not using the refrigerator , may reduce fatalness with CYP2E1C1/C1genotype crowds to have esophagus cancer's risk in Kazakh Xinjiang.