Effects Of Recombinant Human Growth Hormone Combined With Chemotherapy On The Growth Of Bel-7402Human Hepatic Carcinoma Cell Lines In Vitro

Objective: This study aimed at investigating the effect in hepatocarcinoma cellBel-7402cells by combining recombinant human growth hormone with ADM.Methods: Eight groups were assigned as control group, rhGH groups,rhGH+ADM groups and ADM group. Bel-7402cells were cultured with variousconcentrations of rhGH and rhGH+ADM, then cell culture technique, MTTassay and flow cytometry were used to assess the growth rate, growth curve, cellcycle and PI on liver tumor cell Bel-7402.Results: The growth rate in Medium and High concentration of rhGH groupswere significantly increased compared with the controls(P<0.05);ADM groupobviously decrease the Growth of Bel-7402cells(P<0.05); Compared with theADM group,inhibition rate of rhGH+ADM groups were significantly decreased(P<0.05);Growth curve indicated that the growth of rhGH groups were increasedcompared with the control group,especially rhGH2group; Compared withrhGH+ADM groups,growth of ADM group was decreased (P<0.05).The cellcycle assay indicated that the G2/M phase and PI in the rhGH2and rhGH3 groups were obviously increased compared with the control group;G2/M phaseand PI in rhGH+ADM groups were increased compared with ADMgroup(P<0.05).Conclusions: The rhGH stimulated the growth of Bel-7402hepatic carcinomacell line in vitro;rhGH combined with ADM will weaken the inhibitory effect ofthe ADM.