The Experimental Study Of Effects Of Bushengubenfang On Bone Biomechanics And Bone Density In Ovariectomized Rats

Objective： The Experimental Study of Effects of Bushengubenfang on BoneBiomechanics And Bone Density in Ovariectomized Rats.Method: The1056-month-old female Wistar rats (body weight300±20g), wererandomly divided into control group (A n=15); another group made modules (OVXn=90), after successful modeling randomly divided into six groups，namely, modelcontrol group (group B n=15), Xianlinggubao group (group C n=15), the estrogengroup (D group n=15), kidney consolidate high dose group (E group n=15)，kidneyconsolidate prescription dose group (F n=15), kidney consolidate low dosage group(G group n=15). Copy osteoporosis animal model, after the model is successful, thepacket administration. Three months after the administration of all the animals weresacrificed unity (n=6)，remove the first lumbar vertebra, lumbar spine and femurbone second and animal serum, right femur and lumbar spine bone biomechanicalparameters of bone mineral density; ELISA method serum biochemical parameters:rat type1collagen telopeptide cross-linked enzyme, rat procollagen type1amino-terminal peptide cross-linking enzyme, N-terminal peptide of type1collagenNTX, type1collagen C telopeptide CTX, estrogen E， changes in parathyroidhormone PTH，calcitonin, alkaline phosphataseAKP’s.Result:1. BMD and bone mineral content: Compared with the normal group, modelgroup femur bone mineral density and bone mineral content decreased significantly (P<0.05); and model group, the treatment group improved bone mineral density andbone mineral (P <0.05), kidney high, significantly increased in the low dose groupand Xianlinggubao bone mineral density (P <0.01);2. bone biomechanical properties:the model and compared with normal rats, rat femur and lumbar elastic modulus,maximum load, yield strength decreased (P <0.05). Kidney high dose group andmiddle dose group, Xianlinggubao group and model group compared femur abovebiomechanical indicators have improved (P <0.05), compared with the model group,kidney and high dose of rat lumbar spine biomechanics have a role (P <0.05),high-dose group had significantly improved elastic modulus of the lumbar spine (P<0.01). Ovariectomized rats significantly decreased serum CTX, CT, PTH, OCNlevels than the control group compared to normal (P <0.01), kidney consolidategroups (high, medium and low dose) CTX, CT:.3. serum biochemical markers,OCN levels than before treatment improved significantly (P <0.05), which consolidatethe high-dose serum and kidney of rats CTX, CT, PTH, OCN levels weresignificantly higher than the other groups (P <0.01), the CT dose group, OCN levelshave improved significantly (P <0.01), the difference was statistically significant,high, no difference between the dose groups (P>0.01).Conclusion:1. bone mineral density and bone mineral content in ovariectomized rats,bone biomechanical indicators of bone metabolism and blood biochemical parameterswere significantly reduced;2.kidney and restoring order in ovariectomized ratssignificantly improved bone mineral density and bone mineral content；3.kidney andrestoring order to better improve the biomechanical properties of bone inovariectomized rats;4.significant improvement in order to consolidate the kidney to the blood and bone metabolism in ovariectomized rats biochemical indicators.