Preliminary Study On Gardenia Extract To Rat’s Alcoholic Liver Disease With Serum Lipids And TNF-α, IL-6Change Impacts

Objective: To construct the animal model of SD rats with alcoholic liver disease, andexert the gardenia extract synchronous intervention, then through the testing experimentrat’s serum lipids from total cholesterol (TC), triglyceride (TG), cereal third transaminaseamino acid alanine transaminase (ALT), aspartate aminotransferase (AST) levels, thedetermination of the liver index and liver tissue of glutathione peroxidase (GSH-Px) andmalondialdehyde (MDA) content, and then observe the liver tissue tumor necrosis factoralpha (TNF-α), interleukin6(IL-6) gene transcription expression level and rat hepatocytefatty change situation, in order to explore the pathogenesis of alcoholic liver disease andgardenia extractive on its pathological changes impact.Methods: Fifty healthy male SD rats (the age of6-8weeks, weight180-210g.rats),were randomly divided into5groups (n=10), normal control group (control group),alcoholic liver disease model group (model group), gardenia extract low doses ofintervention group (low dose group), gardenia extract dose in the intervention group(middle dose group) and gardenia extract high dose of intervention group (high dosegroup).In addition to the control group rats given ordinary feed and clean drinking water,the rest of the group are fatty forage and56%alcohol every day15ml/kg to fill thestomach, the drug dose group at the same time every day to add low, medium and highdose of gardenia extract lavage, continuous feeding eight weeks. Then the animals wereanesthetized respectively, the preparation of the experimental animal specimens drawn.(1) Took the liver with electronic weighing scales, calculate the liver index;(2) The use of biochemical method to detect the serum TC, TG, ALT and AST, and usethe kits in rat liver GSH-Px and MDA content;(3) By HE staining and immunohistochemical staining were observed by morphologicalchanges of liver tissue, the expression of IL-6and TNF-α positive change indicator;(3) By HE staining and immunohistochemical staining were observed with light microscopemorphological changes in liver tissue and the expression of TNF-α, IL-6positiveindicators of change;(4) extracted the liver tissue of RNA, respectively by fluorescence quantitative RT-PCRmethod in the detection of liver tissue TNF-α and IL-6mRNA level of genetranscription.Results:(1) After eight weeks of veterinary drugs, rat liver index calculation, foundthat increased liver index of model group than the control group (P <0.05), the druggroup of liver index is significantly lower in the model group (P <0.05);(2) The modelgroup rats serum TC and TG contents were significantly higher than the control group (P<0.05); The each drug group compared with model group, serum lipids decreasedsignificantly (P <0.05); Liver function of model group ALT and AST values weresignificantly higher than control group (P <0.05); The liver function each drug groupwere lower than the untreated group (P <0.05); Liver MDA content in model group wassignificantly higher (P <0.05), each drug group MDA significantly lower levelscompared with the model group (P <0.05); GSH-Px content of model group wasobviously lower than the control group (P <0.05), The content of GSH-Px drug groupwas obviously higher than that of model group (P <0.05).(3) HE staining were observedunder light microscope and degree of hepatocyte fatty change of each drug groupcompared with model group rats have obvious improvement; Immunohistochemicalstudy results showed that the model group than the control group in the rat liver tissueTNF-α and IL-6expression increased (P <0.05), the drug group were lower than themodel group (P <0.05).(4)The RT-PCR results showed that the model groups within theliver tissue TNF-α quantity, IL-6mRNA gene expression were significantly higher thanthat of control group, and higher than the drug group (P <0.05).Conclusion:(1) Successfully established SD rats with alcoholic liver damage model,and the application of gardenia extract as an intervention to improve model group rats liverdamage.(2) The model group rats serum lipids TC, TG, ALT, AST and MDA contentincreased, GSH-Px levels decreased;The use of gardenia extract to drug intervention, can make the lipid serum TC, TG, ALT, AST and MDA content decrease, GSH-Px levelsincrease.(3) The model group rats liver tissue of TNF-α higher, IL-6gene transcription andexpression, and use of gardenia extract can make its lower drug intervention. On the whole,serum lipids SD rat model group elevated levels of TC and TG, ALT liver, elevated ASTand MDA levels and GSH-Px was reduced, and liver tissue TNF-α and IL-6genetranscription and expression increased with alcohol related liver damage; Gardenia extractmay be reduced by TNF-α IL-6transcription and expression, and promote fat metabolism,remove free radicals in liver tissue and thus play a role in the treatment of liver.