The Impact Of Maintenance Hemodialysis For TCM Wet Muddy Syndromes With Spleen And Kidney Qi And Regulatory T Eells In Uremia Patients

Objective:Dectecting the levels of regulatory T cells (Treg) in patients on maintenance hemodialysis before and after dialysis, to find out whether dialysis could improve the level of regulatory T cells in patients with uremia. And observing the changes of turbid dampsymptom before and after dialysis, to find out whether dialysis to find out whether the wet muddy symptoms.Method:30cases of CKD Ⅲ-Ⅳ patients (based on the U.S. K/DOQI chronic kidney disease diagnostic criteria) and25cases of patients on maintenance hemodialysiswere selected. All of them comformed to the classification criteria of the Chinese Bianzheng. three-color staining flow cytometry (FCM) were used to detecte CD4+CD25+CD127|。T cells (Treg) levels in peripheral blood. Evaluated the turbid damp symptom scores, including the associated symptoms, tongue, and pulse, before and after hemodialysis.Results①Before hemodialysis, Treg level in CKD Ⅲ-Ⅳ group significantly reduced comparing with normal group (P<0.01), before hemodialysis BUN, P level compared with CKD Ⅲ-Ⅳ group (P<0.05) higher.②There was no significant change in Treg level before and after hemodialysis, P>0.05; but BUN, P levels decreased after hemodialysis (P<0.01).③After hemodialysis, total turbid damp symptom scores improved (P<0.05). Scores of nausea, vomiting, heavy sensation in the limbs and body, poor appetite and indigestion symptom improved (P<0.01) comparing with pre-dialysis.Conclusion:Uremic toxins could lead to lack of Treg; Treg involved in the occurrence and development of uremia; dialysis didn’t improve Treg deficiency. turbid damp symptom improved significantly after dialysis.