Effect Of Rosuvastatin On TLR2and Caspase-3Expression In Subjected To Experimental Intracerebral Hemorrhage In Rat Brain Tissue

objective:in order to explore the possible mechanism that how the rosuvastatin protect nerve tissue,we observe the neuroprotective effect of rosuvastatin and its influence to the expression levels of Toll-like receptors-2(TLR2) and cysteinylaspartate specific proteinases3(caspase-3) in the rats of experimental intracerebral hemorrhage (ICH)。Methodes:To utilize stereotaxic apparatus and infuse Saline into the right basal ganglia to build up shamed operation group (A group,25rats); build up rat experimental ICH model (B group,50rats)by infusing quantited autologous blood into the right basal ganglia;25rats were randomly selected from the group B, given rosuvastatin (10mg/kg/d) gavage treatment,named with ICH rosuvastatin therapy (lOmg/kg)(C group,25rats). After injecting, useing the Garcia’s score method to evaluate neural function defect of the sham operation group, cerebral hemorrhage model group and rosuvastatin statin intervention group at each time point (12h,24h,48h,72h,7d)。 Then the SD rats were killed, neuronal apoptosis was measured at each time point(12h,24h,48h,72h,7d) by tunel method, expression of TLR2alpha and caspase-3 around hematoma in rats’ brain were analyzed by enzyme linked immunohistochemistry (IP)。Results:1. Garcia score showed neurological deficit occured after cerebral hemorrhage,and the symptoms reached a peak at48h.Neural function scores of shamed operation group were higher than ICH group; In addition to the12hours and7days, compared with the rosuvastatin group (C group), neurological deficits of ICH group(B group) were more severe, the results were statistically significant (P<0.05).2. Tunel assay showed that a small amount of tunel positive cells were expressed in shamed operation group(group A) at each point time, while a great amount of tunel positive cells were expressed in ICH group(group B);The expression levels of the tunel positive cells at each point time in ICH group were higher than the shamed operation group; In addition to the12hours, the expression levels of the tunel positive cells at each point time in rosuvastatin intervention group (group C) was lower than the ICH group, the results were statistically significant (P<0.05)。3. Immunohistochemistry showed that a small amount of TLR2and caspase-3positive cells were expressed in shamed operation group(group A) at each point time, while a great amount of TLR2and caspase-3positive cells were expressed in ICH group(group B);The expression levels of TLR2and caspase-3positive cells at each point time in ICH group was higher than the shamed operation group; In addition to the12hours, the expression levels of TLR2and caspase-3positive cells at each point time in rosuvastatin intervention group (group C) were lower than the ICH group, the results were statistically significant (P<0.05)。conclusions:1.Rosuvastatin may reduce the neurological deficit after intracerebral hemorrhage.2. Rosuvastatin may relieve nerve cells apoptosis after cerebral hemorrhage.3.Easing the TLR2and caspase-3expression level, which may be one of the mechanisms that rosuvastatin play a neuroprotective role in cerebral hemorrhage.