Effect Of Capsaicin On Ethanol Induced Acute Gastric Mucosal Injury

bjective: To investigate the effect of capsaicin on ethanolinduced acute gastric mucosal injury in rats,and examine the expression oftransient receptor potential type1ion channel (TRPV1),calcitonin gene relatedpeptide(CGRP),subs nce taP（SP）in gastric antrum and the NO’s content ingastric tissue,and to explore the effect of capsaicin on ethanol induced acutegastric mucosal injury in rats.Methods:SD rats were randomly divided into4groups,group A(the normal control group), group B(the CAP group),groupC(the ethanol group),group D(the CAP and ethanol group),10rats in eachgroup.The detailed experimental method:Rats in group A and C are free accessto food and water,after4weeks,set up the experiment by intragastricadministration,in group A use normal saline for10ml/kg,and group C use75%ethanol for10ml/kg;Rats in group B and D eat the feed which contains CAP1mg/g,after4weeks,set up the experiment by intragastric administration,ingroup B use normal saline for10ml/kg,and group D use75%ethanol for10ml/kg.Then kill the rats2hours later,remove the stomachs,observe the gastricmucosa injury index and use the gastric mucosa for pathologicalexamination;Use the gastric antrum tissue test the expression of TRPV1,CGRPand SP with immunohistochemical method;The residue stomach tissue wereused for testing NO content.Results:1.General states of the rats:During theexperiment,the rats in all groups were in good states.2.Gastric mucosal barrier： (1).The damage of gastric mucosal by naked-eye:The mucosas of group A andgroup B were smooth.Group C’s mucosas were hyperemia,edema,multipleerosions.Group D’s mucosas were hyperemia,edema,but less erosivelesions.The score of gastric mucosal injury (Guth standard modified score) ingroup A,B,C,D were:0.50±0.97,0.80±1.03,14.60±1.78,13.67±1.58.Thegroup C’s score was higher than A,B,D(P<0.05).The group D’ score was higherthan A,B(P<0.05).(2).The damage of gastric mucosal in histopathology:Themucosas of group A and group B were normal epithelial cells.The group C’sgastric mucosal were epithelial damage,shedding,mucosal edema,neutrophilinfiltration.The group D’s gastric mucosal were epithelial cells with mildloss,congestion,edema,and no disturbance of glandular structure and epithelialcell necrosis,inflammatory cell infiltration.Injury of rat gastric mucosapathology points(Masuda standard method) in group A,B,C,D were:0.40±0.70,0.70±1.06,3.90±0.88,2.78±0.83.The group C’s pathological tissuedamage was higher than the group A,B,D(P<0.05),the damage of pathologicaltissue in group D was higher than A,B(P<0.05).3.The expression of TRPVl intissue:The expression leve of TRPVl in A,B,C,D were:1.38±0.39,2.74±0.63,1.42±0.40,2.82±0.51.The expression leve of TRPV1in group B andgroup D were higher than group A and group C(P<0.05).4.The expression ofCGRP in tissue:The expression leve of CGRP in group A,B,C,D were:1.52±0.50,3.22±0.45,1.64±0.51,3.31±0.55,The expression leve of CGRP in groupC were lower than group A,B,D(P<0.05).The expression leve of CGRP in groupA were lower than group B and group D(P<0.05).5.The expression of SP in gastric tissue:The expression leve of SP in group A,B,C,D were:0.42±0.40,1.48±0.43,1.45±0.40,1.53±0.45. The expression leve of SP in group Awas significantly lower than group B group C group D group (P <0.05), theexpression leve of SP in group B,group C,group D were not statisticallysignificant.6.The content of NO in gastric tissue:The content leve of NO in rat’santral zone in group A,B,C,D were:5.58±0.56ug／ml,5.98±0.33ug／ml,4.23±0.51ug／ml,4.63±0.59ug／ml.The quantily leve of NO in rat’s antral zonein group C and group D were significantly lower than group A and groupB(P<0.05).Conclusin:(1)The CAP feed feeding method, enabling the ratstiming, quantitative intake CAP, The method is simple, side effects.(2). Ratsintake with CAP(5mg/kg/d) feed four weeks of normal gastric mucosainjury.(3).Rats intake CAP(5mg/kg/d) four weeks can prevent the acute gastricmucosal injury induced by ethanol.(4).Rats intake CAP(5mg/kg/d) four weekscan increase the expression of TRPV1,CGRP and SP in gastric mucosa.(5).Theprotective role of CAP on gastric mucosa may be related with TRPV1andCGRP,and has no obvious relationship with SP,NO.