Study On The Expression And Correlation Of PTEN And EIF4E In Non-small Cell Lung Cancer

Objective：To examine and compare the expressions of PTEN and eIF4E innon-small cell lung cancer and control non-cancerous lung tissues;To analyzethe relationship of two gene expression and Clinicopathological features; Toexplore the correlation between two genes and the malignancy degree ofNSCLC,This study was helpful for consummating the mechanism of lymphnode metastasis and differentiation in NSCLC, screening tumor associatedmarkers, improving detection level, finding new theory and therapeutic targetin NSCLC.Method:Forty-eight NSCLC patients who meet the standards were selectedrandomly in the Affiliated Hospital of Chengde Medical College fromDecember2009to September2011.We used reverse transcription polymerasechain reaction and immunohistochemical to detecte PTENmRNA,eIF4EmRNA and their corresponding proteins in NSCLC tissues and controlnon-cancerous lung tissues.Statistical analysis was carried out to analyse thecorrelation between the two markers and biocharacteristics binding with thecase history and the relationship of two genes.Results:1The relative expression amount of PTENmRNA were0.336±0.112inNSCLC tissues and0.872±0.291in non-cancerous lung tissues. There wassignificant difference in two groups （P＜0.05）. The expression ofPTENmRNA were significant lower in patients with lymph node metastasisthan in those without lymph node metastasis（P＜0.05）.Compared with thewell and moderate differentiation groups, the poor differentiation group hadlower PTENmRNA expression level（P＜0.05）. The relative expression amount of eIF4EmRNA were0.829±0.215inNSCLC tissues and0.243±0.167in non-cancerous lung tissues. There wassignificant difference in two groups （P＜0.05）. The expression ofeIF4EmRNA were significant higher in patients with lymph node metastasisthan in those without lymph node metastasis（P＜0.05）.Compared with thewell and moderate differentiation groups, the poor differentiation group hadhigher eIF4EmRNA expression level（P＜0.05）.2The expression rate of immuno-positive staining for PTEN proteinwere70.83%（34/48）in control non-cancerous lung tissues and27.08%（13/48）in NSCLC tissues. There was significant difference in two groups（P＜0.05）. The expression of PTEN were significant lower in patients withlymph node metastasis than in those without lymph node metastasis（P＜0.05）.Compared with the well and moderate differentiation groups, thepoor differentiation group had lower PTEN expression level（P＜0.05）.The expression rate of immuno-positive staining for eIF4E protein were31.25%（15/48）in control non-cancerous lung tissues and68.75%（33/48）in NSCLC tissues. There was significant difference in two groups（P＜0.05）.The expression of eIF4E were significant higher in patients with lymph nodemetastasis than in those without lymph node metastasis（P＜0.05）.Comparedwith the well and moderate differentiation groups, the poor differentiationgroup had higher eIF4E expression level（P＜0.05）.3There was a negative correlation between the expression of PTEN andeIF4E in NSCLC（P＜0.05）.Conclusions:1The expressions of PTEN and eIF4E in NSCLC tissues and non-cancerous lung tissues had a significant difference, indicating there was aclosed relationship between the two genes and NSCLC.2In NSCLC,the expression of PTEN were significant lower in patientswith lymph node metastasis than in those without lymph nodemetastasis.Compared with the well and moderate differentiation groups, thepoor differentiation group had lower PTEN expression level; The expression of eIF4E were significant higher in patients with lymph node metastasis thanin those without lymph node metastasis.Compared with the well and moderatedifferentiation groups, the poor differentiation group had higher eIF4Eexpression level.It indicates that the expressions of PTEN and eIF4E inNSCLC had significant correlations with lymph node metastasis and tumordifferentiation.3The expressions of PTEN and eIF4E were negative relevant.