Naringin Regulation The Expression Of COX-2in Human Ovarian Carcinoma Cell Line SKOV3

Objective：To investigate effects of naringin on expression of COX-2mRNA and protein ofHuman ovarian cancer cell line SKOV3.Methods：1、SKOV3cells were cultured in vitro and divided into five groups randomly,namely control group, naringin group(10μmol/L),naringin group(20μmol/L), naringingroup (40μmol/L) and celecoxib group(80μmol/L).2、The inhibition effects of naringin on cell-proliferation were determined byMTT method.3、The expression of COX-2mRNA were evaluated by real time quantitativePCR after Forty-eight hours.4、The expression of COX-2protein were evaluated by Western blot afterForty-eight hours.Results：1、Naringin could inhibit the growth of SKOV3cells, and with the increase ofthe dosage and time，the tumor growth inhibition rate increased significantly.The24hgrowth inhibition rates of naringin groups (10umol/L，20umol/L，40umol/L) were35.78%,59.63%,76.15%,and the48h growth inhibition rates were45.36%,67.12%,85.50%,and the72h growth inhibition rates were47.09%,75.87%,90.32%.Respectively，each was significantly higher than that of the control group(P<0.05).2、Compared with control group(1.094±0.053)，the expression of COX-2mRNAin naringin and celecoxib groups were0.828±0.006,0.753±0.011,0.412±0.216,0.321±0.017，which were significantly lower than that of the control group(P<0.05)，especially the40umol/L group and celecoxib group(P<0.01).3、Compared with control group（1.7325±0.0826），the expression of COX-2protein was higher by10μmol/L naringin group（1.7925±0.0880），but there was nostatistical difference. However, when SKOV3cells were treated by other dosage ofnaringin and celecoxib, the expression of COX-2protein were down-regulated significantly (1.2225±0.0822,1.2725±0.07632,0.3075±0.0977；P<0.05).Conclusions：The appropriate dosage of naringin can inhibit SKOV3cells growth in vitro, andcan down-regulate the expression of COX-2mRNA and protein.