Application Of TaqMan Real-time PCR Assay For Detecting Cervical Lesions Patients’ High-risk Human Papillomavirus

Cervical cancer is one of the common malignant tumors of female reproductivesystem, is a global public health problem. Cases in2008the global new cases ofcervical cancer is about529800,255100deaths, of which85%new cases indeveloping countries. Research suggests thatcervical cancer is composed of cervical intraepithelial neoplasia (CIN) graduallyevolved, and cervical cancer’s occurrence, development and comprehensive effects ofvarious risk factors result, the through sexual transmission type cervical infection withhigh-risk human papilloma virus (HR-HPV) is one of the risk factors of cervicalcancer and precancerous lesion. HR-HPV infection can be by medical intervention toprevent and cure, therefore, early detection of HR-HPV infection and to take activeintervention is the key to the prevention of cervical cancer and precancerous lesions.Objective:Detection of high-risk human papillomavirus DNA real-time fluorescencequantitative PCR method using TaqMan, combined with the papilloma virus infectionin cervical lesions and high-risk human pathological diagnosis, to provide referencedata for early screening of cervical cancer and cervical intraepithelial neoplasia.Method:511women with cervical diseases for a maternity hospital treatment centerfrom January to December2012in Changchun city were slected in this research,high-risk human papilloma virus DNA by real-time fluorescent quantitative PCRmethod to detect TaqMan in511cases of cervical disease in secretion, collection,collation on the test results, combined with high-risk human infection uterine cervicallesions and high-risk human pathological diagnosis. Result:HR-HPV DNA by real-time fluorescent quantitative PCR method to detectTaqMan in511cases of cervical disease secretion in chronic cervicitis patients, thepositive rate of HR-HPV was14.55%, CIN Ⅰ patients infected with the positive rateof HR-HPV was33.33%, CIN Ⅱ infection in patients with the positive rate ofHR-HPV was48.42%, CIN Ⅲ patients infected with the positive rate of HR-HPVwas80.85%, CIN II, CIN III patients with HR-HPV infection rate was significantlyhigher than that of CIN Ⅰ patients and chronic cervicitis patients, the difference wasstatistically significant (P<0.05). Analysis of HR-HPV DNA copy number ofnumerical distribution, results show that the HR-HPV DNA copy number and thedegree of cervical lesions had no significant difference (P>0.05). Analysis of patientswith cervical diseases among each age group the positive rate of HR-HPV infection,the results showed that the positive rate of HR-HPV infection among age groups hadno significant difference(P>0.05).Conclusion:Detection of HR-HPV DNA real-time fluorescence quantitative PCR methodTaqMan has good reproducibility, which provided reference for application of themethod to detect HR-HPV DNA for cervical cancer. The occurrence, developmentand HR-HPV cervical lesions associated with infection, aggravating the degree ofcervical lesions, the positive rate of HR-HPV infection significantly increased.HR-HPV DNA copy number and the degree of cervical lesions had no significantdifferences, with the severity of cervical lesions, no significant increase in HR-HPVDNA copy number. Patients with cervical diseases among each age group the positiverate of HR-HPV infection had no significant difference.