The Study On The Expression Of Centrosome Abnormalities In Cervical Lesions And Its Correlation To High-risk Human Papillomavirus Infection

ObjectiveThis study attempted to explore the possible mechanism of human papillomavirus infection leads to the cervical cancer by investigating the centrosome abnormalities in cervical lesions and its correlation to high-risk human papillomavirus infection.MethodsChoose 89 cases of cervical lesions were diagnosed by pathology in the First Affiliated Hospital of Jinan University from November 2008 to August 2010, including 46 eases of cervical intraepithelial neoplasia(11 cases CINⅠ,12 cases CINⅡ,23 cases CINⅢ),43 cases of cervical squamous cell cancer, and another 20 cases of normal cervical tissue from Uterine benign lesions (uterine fibroids, adenomyosis).All patients tested HPV-DNA by HybriMax before any therapy. Besides, Cervical tissue specimens which obtained by colposcopic biopsy, cervical conization, hysterectomy were investigated for centrosome status by using indirect immunofluorescence staining with an antibody against y-tubulin was used to label centrosome. The amount and form of centrosome observed by Fluorescence microscope. The results were analyzed by SPSS13.0.Results1. In this study, the most common type of HPV infection is 16, and followed by the 58 and 33. The proportion of High-risk HPV in the normal cervical, CIN, cervical squamous cell carcinoma, respectively 5%,8.26%,93.02%, the trend was gradually increasing. The proportion of High-risk HPV in CINⅠ, CINⅡ, CINⅢ, respectively 45.45%,83.33%,91.30%, the trend was gradually increasing. HR-HPV has no relation with clinical stage and histological grade of cervical squamous cell cancer.2. Normal cervical epithelium showed the centrosome with normal number and size, while CIN and cervical squamous cell cancer were found in varying degrees of centrosome abnormalities, centrosome abnormalities showed abnormal centrosome morphology or number increased.3. The proportion of abnormal centrosome of CINI, CINII, CINIII, respectively(0.25±0.20)%, (1.34±0.73)%, (2.70±1.94)%, the difference was statistically significant. The proportion of abnormal centrosome of the stageⅠ,Ⅱ,Ⅲof the cervical squamous cell carcinoma, respectively (3.26±0.96)%, (5.13±2.31)%, (5.71±2.11)%, the difference was statistically significant. The proportion of abnormal centrosome of the High, medium, poorly differentiated respectively (3.86±1.21)%, (4.10±1.85)%, (5.66±2.63)%, the difference was no statistically significant.4. The proportion of abnormal centrosome of CIN and cervical squamous cell carcinoma respectively (1.76±1.74)% and (4.46±2.08)%, the difference was statistically significant. Abnormal centrosome rate increased gradually with the increase of CIN grade and clinical stage of cervical squamous cell cancer and the two showed a significant positive correlation.5. In the group of CIN, the proportion of abnormal centrosome of the high-risk HPV infection is higher than that of HPV-negative, the difference was statistically significant. In the group of cervical squamous cell carcinoma, the proportion of abnormal centrosome of the high-risk HPV infection is higher than that of HPV-negative, the difference was statistically significant. According to bivariate correlation analysis, in cervical lesions, abnormal centrosome rate and high-risk HPV infection showed a significant positive correlation.Conclusion1. High-risk HPV infection is correlated with the progression of CIN and cervical cancer. The characterization of HPV-DNA typing can provide significant theoretical and practical basis for screening high risk population and vaccine development.2. Centrosome abnormalities is one of the characteristics of cervical squamous cell cancer and it can serve as a complementary biomarker to cytological examination. 3. Centrosome amplification was an early event and that might play a role in the progression of cervical squamous cell cancer.4. In the process of development of cervical squamous cell carcinoma, centrosome abnormalities and high-risk HPV infections are closely related. High-risk human papillomavirus infection may lead to cervical cancer through inducing centrosome amplification.