The Content And Significance Of P2X₇Receptor And VEGF In Vitreous Fluid Of Patients With Proliferative Vitreoretinopathy

Objective The experiment to detect the P2X₇Receptor and vascular endothelialgrowth factor（VEGF） levels in vitreous fluid of patients with proliferativevitreoretinopathy （PVR）, proliferative diabetic retinopathy （PDR） and normal control byenzyme-linked immunosorbent assay.To analyze the correlation between the purinergicreceptor P2X₇and VEGF in proliferative vitreous retinopathy.Methods According to the patient’s general condition, eye-related history andfundus examination, the experimental group are as follows:①patients with proliferativevitreous retinopathy （PVR）, a total of15cases （15eyes）, male11cases, female4cases, aged35-72years old, mean age was48.3±10.6years old;②2-type diabetesmellitus patients accompanied proliferative diabetic retinopathy （PDR）, a total of18cases （20eyes）, male6cases, female12cases, aged48-75years old, mean age was64.3±7.2years old.③patients with idiopathic macular hole,10cases （10eyes）, as anormal control group, male3cases, female7cases, aged46-71years old, mean age was59.3±12.1years old. A total of43cases（45eyes）.There was no statistically differencebetween groups in terms of age, gender and so on （P＞0.05）.15patients in group PVRincluded B grade2cases,C grade10cases,D grade3cases （classification according to theretina standard classification society, published in1983）, average history was27days（9-75days）;18patients in group PDR included Ⅳ period8cases, Ⅴ period5cases,Ⅵ period5cases （7eyes），（installment standards with reference to the Chinesemedical association in1984diabetic retinopathy）, with average12years （8-21years）history of diabetes mellitus, such as see things black shade, see things disappear, eyesshadow float and so on, ocular history with an average of5months （2-17months）. Allpatients were strictly meet the vitreous cut surgical indications, before the surgery, signthe operation consent and sample collection informed consent,routine vitrectomy operation,put0.2-0.3ml intraoperative vitreous humor samples into1.5ml Eppendorffcentrifuge tube. Then the samples were preserved into-70℃refrigerator. After thesamples were collected complete by double antibody sandwich enzyme-linkedimmunosorbent assay （ELISA）,to detecte the content of purinergic receptors P2X₇andVEGF in the vitreous fluid of group PVR,group PDR, and normal control group.Anaylze the correlation between purinergic receptors P2X₇and VEGF in group PVRand group PDR respectively.Results The content of purinergic receptors P2X₇in the vitreous fluid of groupPVR was500.45±90.37pg/ml, while the content was369.72±116.24pg/ml in groupPDR. Both of them were significantly higher than the normal control group145.05±76.92pg/ml, the differences were statistically significant （P<0.01）, the contentof purinergic receptors P2X₇in group PVR was higher than that in group PDR, thedifference was statistically significant （P<0.05）. The content of VEGF in the vitreousfluid of group PVR was249.64±36.90pg/ml, while the content was1289.69±295.77pg/ml in group PDR. Both of them were significantly higher than the normal controlgroup145.44±38.58pg/ml, the differences were statistically significant （P<0.01）, thecontent of VEGF in group PVR was lower than that in group PDR, the difference wasstatistically significant （P<0.01）.The purinergic receptors P2X₇content and VEGFcontent were respectively positively correlated in the vitreous fluid of PVR group andPDR group.（R respectively.0.963,0.946, P <0.05）.Conclusion1.The purinergic receptor P2X₇content in vitreous fluid of PVRpatients increased significantly, and the increase amplitude is greater than VEGF,suggesting that P2X₇may play a regulatory role in PVR occurrence and development，and its effect of prompted PVR progression is stronger than VEGF.2.The purinergic receptor P2X₇content in vitreous fluid of PDR patients increased,and the increase amplitude is less than VEGF, suggesting that P2X₇may be synergisticVEGF to play a regulatory role in PDR occurrence and development.3. PVR and PDR, the content of purinergic receptor P2X₇and VEGF have somedegree of increased relative to the normal, suggesting that they in proliferativeretinopathy may play a certain role in promoting.