| In recent years, Cerebral infarction has become a common and frequ-ently occurring disease, seriously affecting people’s life. After cerebral ischemia, neuronal cell death is mainly characterized as two forms, necrosis and apoptosis, the necrosis (necrosis) of neuron in central of ischemia area is not reversible, but in the penumbra of ischemic, neural cells go to death in the form of apoptosis (apoptosis). Therefore, how to reverse the process of apoptosis and save the penumbra of ischemic is a hotspot of curing cerebrovascular disease.At present, thrombolytic and neuroprotective are the two therapeutic measures of curing ICVD. Thrombolytic therapy is to save reversible brain tissue and reduce the infarct size and improve the prognosis through restoring the blood reperfusion as early as possible and reconstructing the collateral blood circulation. However, the reperfusion caused by the recanalization of blood vessels may induce apoptosis of nerve cell and infarction volume expansion. Therefore, the neuroprotection has become more and more important. Current neuroprotective drugs mainly include Ca2+antagonist, glucocorticoids, free radical scavengers, NOS inhibitors...etc, but most of the drugs doesn’t perform well in treatment and always cause adverse reaction. Instead Chinese herbal medicines represented by the Decoction of Bu yang Huan wu perform well in treatment of ischemic cerebral vascular disease and outstanding in less adverse reaction would have a very extensive use in the future. Clinical verified chinese medicine compound preparation of Kangnaoye, a derivative medicine of the Bu yang Huan wu Decoction, can supplement Qi and promote blood circulation by removing blood stasis, and clear heat, and calm the liver and the scareness. Through making cerebral ischemia reperfusion model, this experiment is to observe the effect of Kangnaoye on regulatory protein Fas and protein FasL of upstream and the expression of executive protein Caspase-3of downstream while Fas apoptosis, thus to discuss the mechanism of Kangnaoye’s function, and then to sets up a theory basis of deeper level for clinical application.The module of middle cerebral artery occlusion (MCAO) of rats was introduced by the suture method, Rats were given reperfusion by3h,6h,12h,24h,48h respectively,2h after cerebral ischemia. The192male SD rats were randomLy divided into six groups:sham-operated group; model (ischemia and reperfusion) group, KangNaoYe group with high dose, KangNaoYe group with medium dose, Kang NaoYe group with low dose, Edaravone group, The sham-operated group has12male SD rates respect-ively, the rest group has36male SD rats respectively. It’s to observe the Neurological evaluation, pathological change of rats’brain tissues using the method of HE Staining and the volume change of cerebral infarction in rats by the method of TTC Staining. The immunohistochemis-try were used to measure the expression of Fas, fasL and Caspase-3, and the In situ hybridization were used to measure the expression of FasmRNA, FasL mRNA and Caspase-3mRNA.Compared with shamed group, the symptom of Neurological impair-ment, the volume of cerebral infarction and the interstitial edema around neurons were very apparent after the reperfusion in rats of cerebral ischem-ia in model group; the expression of Fas, FasL, Caspase-3protein and gene rose significantly. The difference was significant (P<0.01). After the reper-fusion of in rats of cerebral ischemia, it could be noted in each group with administration of KangNaoYe and Edaravone that the symptom of Neurological impairment was eased and volume of cerebral infarction reduced and neuron swelling mitigated and the expression of Fas, FasL, Caspase-3protein and gene decreased to different degrees, the difference was significant in comparison with the model group(P<0.05or P<0.01); Compared with Edaravone group, KangNaoYe group with medium and high dose could reduce the impairment evaluation of nervous system and the volume of cerebral infraction and the pathological change of brain in varying degrees. The expression of Fas, FasL, Caspase-3protein and gene was apparently reduced, difference was significant (P<0.05or P<0.01). However, there wasn’t significant difference between KangNaoYe group of medium dose and high dose, which are much better than the low dose group (P<0.01).In a summary, KangNaoYe can ease the injury induced by cerebral ischemia. Its mechanism is to adjust the expression of executive protein Caspase-3of downstream, and to block waterfall reaction caused by apoptosis and apoptosis of nerve cell possibly through inhibiting the early expression of Fas, FasL protein. |
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