| Objective: The bacterial infections are frequent in cirrhosis, the maincause of spontaneous bacterial peritonitis is bacterial translocation from bowel.Deficiencies in local host immune defences, impairment in permeability of theintestinal mucosal barrier, intestinal bacterial overgrowth are the majormechanisms postulated to favour bacterial translocation in cirrhosis. Intestinalbarrier plays an important role in maintaining physical healthy by acting as thefirst barrier against bacteria and bacterial products translocation. Bowelfunction is one of the most important factor to determine the prognosis ofpatients, it is directly related to the complications of patients. Bacterialtranslocation and inflammatory mediators are the important factors that lead toinjury of intestinal mucosal barrier. Lipopolysaccharide(LPS) can induceactivation of Kupffer cells to produce pro-inflammatory mediators, plays animportant role in the development and progression of liver cirrhosis.In thisstudy, by observing the effect of Sacchromyces boulardii on intestinal tissuemorphology and the effects of inflammatory cytokines, explore the protectiveeffect and its possible mechanism of Sacchromyces boulardii on intestinalmucosal barrier of rats with cirrhosis.Method:1Animal model: Healthy male S-D rats (n=30) were divided into threegroups at random: control group (C group), model group(M group),treatmentgroup （Z group）.40%CC14olive oil was administered parenterally on theback of rats at a dose of o.5ml/100g body weight for the first time, after thatthe dose was changed to0.3ml/100g twice a week for12weeks, and the onlydranking water was10%ethanol solution. After12weeks, Sacchromycesboulardii formulations was administered to rats75*108CFU/kg·d﹣1in Cgroup by gastric irrigation for14days, A group and B group rats were given the same saline.2By light microscope observed pathological changes of rat intestinaltissue and liver histology.3Assay endotoxin levels of plasma in endpoint chromo-genic TALmethod.4Assay serum TNF-α, IL-6levels in radioimmunoassay method.5Using spectrophotometry method assay activity of diamine oxidase ofintestinal tissue.6Using automatic biochemical analyzer assay serum ALT, AST andalbumin levels.Results:1Ileal mucosa histopathological examination: Observed under lightmicroscope, villus of intestinal mucosa neatly arranged, highly consistent,complete mucosal and villus in the C group; villus of intestinal mucosa withdisordere, sparse, edema, intestinal mucosal with atrophy, part of epithelialcell with necrosis, off, and inflammatory cell infiltration in the M group;compared with the C group, height and thickness of ileal mucosa of rats in theM group, become smaller, thinner. Lesion of intestinal mucosal in the Z groupwas significantly reduced than the M group, villus of intestinal mucosa neatlyarranged, mild villous edema, no obvious epithelial cell loss, inflammatorycell infiltration reduced; after application of Sacchromyces boulardii theheight and thickness of intestinal mucosa obviously improved.2The plasma levels of endotoxin: Concentration of endotoxin of plasmain the M group (0.251±0.011) was significantly higher than that of the Cgroup(0.055±0.05), and there was significantly difference between two groups(P<0.01); Concentration of endotoxin in Z group(0.168±0.012) wassignificantly decreased than that of the M group, the difference had statisticalsignificance (P<0.01), concentration of endotoxin in Z group compared withthe C group the difference had statistical significance (P<0.01).3The content of serum TNF-α and IL-6: The concentrations of serumTNF-α and IL-6in M group(2.37±0.14,214.51±14.13） were significantly higher than that of the C group(0.64±0.12,106.63±9.49), the difference hadstatistical significance (P<0.01); the concentrations of TNF-α and IL-6in Zgroup(1.59±0.13,176.45±10.81) were significantly decreased than that of theM group, the difference had statistical significance (P<0.01), concentrationsof serum TNF-α and IL-6in Z group compared with the C group thedifference had statistical significance (P<0.01).4Activity of diamine oxidase of intestinal tissue: The activity of diamineoxidase of intestinal tissue in the M group(0.432±0.074) was significantlylower than that of the C group(0.728±0.065), the difference had statisticalsignificance (P<0.01); in the Z group(0.548±0.053) it was significantly higherthan that of the M group, the difference had statistical significance (P<0.01);the difference between the Z group and the C group had statistical significance(P<0.01).5The levels of serum ALT, AST and albumin: The concentrations ofserum ALT and AST in M group(133.89±8.09,176.92±10.94) weresignificantly higher than that of the C group(36.73±6.95,41.35±10.07), thedifference had statistical significance (P<0.01), the concentrations of serumalbumin in M group(14.50±1.21) was lower than that of the Cgroup(23.99±1.92); the concentrations of ALT and AST in Z group weresignificantly decreased than that of the M group, the difference had statisticalsignificance (P<0.01), the concentrations of serum albumin in Z groupcompared with M group the difference had no statistical significance.concentrations of serum ALT, AST and albumin in Z group compared with theC group the difference had statistical significance (P<0.01).Conclusions:1Intestinal mucosa of rats with cirrhosis exist a certain degree of injury.2Sacchromyces boulardii has a protective effect on intestinal mucosalbarrier in rats with liver cirrhosis. |
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