Experimental Study On The Dynamid Expression Of TGFβ1and TGFβ2in The Model Of Bulbar Conjunctival Filtering Bleb In Rats

Objective:To establish a model of conjunctival filtering bleb in rats,and observe the expression of transforming growth factorβ1(TGFβ1)and transforming growth factorβ2(TGFβ2) in the process of filtering bleb scarring, then preliminarily discuss the impact of TGFβ1and TGFβ2in the process of filtering bleb scarring after glaucoma filtering surgery.Methods:Forty healthy and adult male SD rats were chose. Five rats were randomly selected as the normal control group and don’t accepted any processing. Conjunctival and subconjunctival organizations of the five rats’ right eyes were taken for western blot detection.Observe the expression of TGFβ1and TGFβ2in normal organizations. The rest of the35rats were chosen as the experimental group and were randomly divided into seven groups in average. Models of conjunctival filtering bleb were built after drainage tubes were successfully put into their right eyes. The filtering bleb formations of the experimental group were observed, and the intraocular pressure fluctuations were monitored after the surgery. The rats of the7groups were killed on1day、3days、5days、7days、14days、21days、28days after surgery in turn. Conjunctival and subconjunctival organizations of the35rats’right eyes were taken for western blot detection.Observe the expression of TGFβ1and TGFβ2on each specific times.Results:1.Different extents and obviously bulgy filtering blebs can be formed in all the eyes of the rats on the first day after surgery. All the filtering blebs can maintain7-22days,and can exist13days on average.2.All the Intraocular pressures of the eyes declined obviously right after surgery, then slowly rose. The Intraocular pressures returned to preoperative level on7days after surgery on average, and Keep to the end of the observation period. Semi-quantitative statistics results of western blot showed that TGFβ1began to rise on the first day after surgery, and kept rising to the peak on5days、7days after surgery, then gradually reduced until28days after surgery, but still higher than the control group. there was Significant statistical different between the two groups (F=431.918,P<0.01).TGFβ2began to rise on the first day after surgery, and kept rising to the peak on7days after surgery, then gradually reduced until28days after surgery, but still higher than the control group. There was Significant statistical different between the two groups (F=674.323,P<0.01)Conclusions:Drainage tube implanting to anterior chamber can establish the rat model of the conjunctival filtering bleb. TGFβ1and TGFβ2took part in the process of conjunctival and subconjunctival filtering bleb scarring.